| 原生质体融合构建耐高温高产酒精酵母 |
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| 引用本文： | 路玲玲,王敏,朱会霞,孙金旭,檀建新,张伟.原生质体融合构建耐高温高产酒精酵母[J].食品科学,2007,28(4):231-236. |
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| 作者姓名： | 路玲玲 王敏 朱会霞 孙金旭 檀建新 张伟 |
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| 作者单位： | 河北农业大学食品科技学院生物工程系； 衡水学院生命科学系； 河北农业大学食品科技学院生物工程系 河北 保定 071001； 河北 衡水 053000； 河北 保定 071001； |
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| 摘 要： | 选择化学药物和紫外灭活单亲原生质体为遗传标记,以0．01%的三唑酮和紫外照射h-1原生质体2min为遗传标记,采用四因素三水平正交试验确定了融合子LZ-3的浓醪酒精发酵工艺。浓醪发酵的最佳工艺条件是:料水比为1:2,糖化酶用量为400U/g原料,接种量为9%(V/V),初始pH值为5．5,在此条件下,以玉米粉的糖化醪为培养基,发酵72h,LZ-3的酒精产率为9．0%。
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| 关 键 词： | 原生质体 融合 耐高温 高产酒精酵母 |
| 文章编号： | 1002-6630（2007）04-0231-06 |
| 修稿时间： | 2006-01-16 |
Building Thermotolerant High-alcohol-producing Yeast by Proplast Fusion |
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| LU Ling-ling,WANG Min,ZHU Hui-xia,SUN Jin-xu,TAN Jian-xin,ZHANG Wei.Building Thermotolerant High-alcohol-producing Yeast by Proplast Fusion[J].Food Science,2007,28(4):231-236. |
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| Authors: | LU Ling-ling WANG Min ZHU Hui-xia SUN Jin-xu TAN Jian-xin ZHANG Wei |
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| Affiliation: | 1.Department of Biology Engineering,College of Food Science and Technology,Agricultural University of HeBei,Baoding 071001,China; 2.Department of Life Science,Hengshui University,Hengshui 053000,China |
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| Abstract: | As chemical drugs and UV used to exterminate the single parent protoplasts were choosen as hereditary markers, with 0.01% triadimefon content and irradiated by UV 2 rain,the optimum conditions of the orthogonal test are:33.3%(W/V) concentration of crude corn in fermenting material,400 U/g of glucoamylase(per gram of raw corn),9%(V/V)inoculum of yeast, and initial pH 5.5.The orthogonal test indicated that the fusion strain LZ-3 produces the highest amount of alcohol,or 9.0% alcohol after incubation at 42℃for 72 hours. |
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| Keywords: | protoplast fusion thermotolerant high-alcohol-producing yeast |
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