玉米秸秆糖醇发酵产丁二酸及表征

以玉米秸秆糖醇液为原料，考察产琥珀酸放线杆菌（Actinobacillus succinogenes）X-1对不同单一碳源的同化能力，并验证产琥珀酸放线杆菌可同化利用玉米秸秆糖醇液。利用Box-Behnken中心组合设计试验，通过响应面分析法优化发酵工艺参数为：玉米秸秆糖醇液初始还原糖质量浓度42.81 g/L、酵母膏质量浓度12.53 g/L、缓冲剂MgCO3质量浓度15.90 g/L，经5 L发酵规模实验，发酵周期48 h，丁二酸产率为85.1%，还原糖利用率为85.4%。经红外光谱和核磁共振表征其发酵产物为生物基丁二酸。

Fermentation and Characterization of Succinic Acid from Sugar-Alcohol Solution of Corn Stover

In this work, the sugar-alcohol solution of corn stover was fermented to produce succinic acid. The carbon
assimilation ability of Actinobacillus succinogenes X-1 was investigated using different carbon sources and the strain was
verified to be able to assimilate the sugar-alcohol solution to produce succinic acid. A Box-Behnken design was used to
optimize three medium components by response surface methodology. The optimum medium was obtained by adding
12.53 g/L yeast extract as a nitrogen source, and 15.90 g/L MgCO3 as a pH buffer to the sugar-alcohol solution containing
42.81 g/L reducing sugar. After 48 h of fermentation in a 5-L fermentor, the yield of succinic acid was 85.1% and the
utilization rate of reducing sugar was 85.4%. The crystal of the fermentation product was obtained by acidic crystallization
and succinic acid was characterized using infrared spectroscopy and nuclear magnetic resonance.