| 苦瓜果实中多糖的分离纯化及性质分析 |
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| 引用本文： | 刘国凌,宁正祥,郭红辉.苦瓜果实中多糖的分离纯化及性质分析[J].食品科学,2010,31(3):30. |
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| 作者姓名： | 刘国凌 宁正祥 郭红辉 |
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| 作者单位： | 韶关学院食品科学与工程系;华南理工大学轻工与食品学院; |
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| 基金项目： | 国家自然科学基金项目(30800913) |
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| 摘 要： | 以苦瓜果实为材料,经热水抽提、乙醇沉淀和Sevag法去蛋白后获得了苦瓜果实粗多糖(MCP)。MCP经DEAE-纤维素柱离子交换柱层析分离得到4个级分MCP-A、MCP-B、MCP-C和MCP-D。MCP-A经Superdex G-100柱进一步纯化得到苦瓜果实多糖MCP-A1组分。MCP-A1经高效凝胶渗透色谱法(HPGPC)鉴定为均一组分,测定其平均分子量为93577D。经过对其理化性质鉴定表明,MCP-A1不含蛋白质、核酸,含有糖醛酸,为非淀粉类多糖。红外光谱扫描结果表明,其具有典型的多糖吸收峰。
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| 关 键 词： | 苦瓜 多糖 分离 纯化 性质 |
Isolation and Characterization of Polysaccharides from Momordica charantia L. Fruits |
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| LIU Guo-ling,NING Zheng-xiang,GUO Hong-hui.Isolation and Characterization of Polysaccharides from Momordica charantia L. Fruits[J].Food Science,2010,31(3):30. |
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| Authors: | LIU Guo-ling NING Zheng-xiang GUO Hong-hui |
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| Affiliation: | LIU Guo-ling1,NING Zheng-xiang2,GUO Hong-hui1(1.Department of Food Science , Engineering,Shaoguan University,Shaoguan 512005,China,2.College of Light Industry , Food Sciences,South China University of Technology,Guangzhou 510146,China) |
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| Abstract: | Crude polysaccharides(MCP) were extracted from dried fruits of Momordica charantia L.through sequential steps of hot water extraction,ethanol precipitation and Sevag protein removal and purified/fractionated by DEAE-cellulose-52 exchange chromatography to obtain four fractions(MCP-A,MCP-B,MCP-C and MCP-D).MCP-A1 with high purity was obtained through further purification of MCP-A by Superdex G-100 gel filtration chromatography and its purity was identified by high performance gel permeation chromatography(HP... |
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| Keywords: | Momordica charantia L polysaccharide isolation purification characterization |
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