响应面优化微波辅助乙醇提取蜂胶黄酮工艺优化

对蜂胶中黄酮类化合物提取工艺条件进行优化。采用单因素试验分析微波时间、微波功率、乙醇体积分数、浸提温度、浸提时间、液料比6个因素对总黄酮得率影响，根据中心组合试验设计原理，采用四因素三水平响应面分析法进行响应面试验，并对各个因素的显著性和交互作用进行分析。结果表明，最优工艺条件为微波时间70s、微波功率282W、乙醇体积分数80%、提取温度77℃、浸提时间12h、液料比25:1(mL/g)，在此条件下，蜂胶总黄酮得率为25.08%。

Optimization of Microwave-Assisted Extraction of Flavonoids from Propolis by Response Surface Methodology

This paper reports the optimization of process conditions for microwave-assisted extraction of flavonoids from propolis using response surface methodology. The effects of six operating parameters including microwave treatment time, microwave power, solvent composition (ethanol concentration), temperature, time and solvent-to-solid on total flavonoid yield were investigated through one-factor-at-a-time experiments. The significance and interaction effects of microwave treatment time, microwave power, ethanol concentration and temperature were analyzed by response surface analysis based on a three-level central composite design. The optimal microwave-assisted extraction conditions for total flavonoids were determined as follows: 70 s microwave treatment at 282 W followed by extraction with 80% ethanol aqueous solution at a solvent-to-solid of 25 mL/g and 77 ℃ for 12 h. Under these conditions, the yield of total flavonoids from propolis was up to 25.08%.