加速溶剂提取法快速分析乌龙茶内含呈香组分

加速溶剂提取法（Accelerated solvent extraction,ASE）是近年来最先进的样品前处理技术之一,具有快速高效等特点.本研究应用ASE技术对凤凰单枞乌龙茶（Camellia sinensis）的呈香组分进行富集,设置最优条件是使用40ml乙酸乙酯为提取溶剂,提取压力9MPa,提取温度120℃,可对10.00g乌龙茶样品的香气成分有效提取,提取时间仅为5min;结合气质联用仪分析鉴定.结果表明采用ASE富集凤凰单枞乌龙茶香气组分,从桂花香型凤凰单枞乌龙茶中检测出63种香气组分,其中较高的组分有芳樟醇、橙花叔醇、壬酸、癸酸、香叶醇、十六烷和3,7-二甲基-1,5,7-辛三烯-3-醇.ASE法可对乌龙茶内含香气组分进行无损富集和快速检测,避免茶叶内含不稳定的香气组分被破坏,减少茶叶香气组分检测的误差和不稳定性.

Application of Accelerated Solvent Extraction on the Oolong Tea (Camellia sinensis cv. Fenghuangdancong) Aroma Analysis

Oolong tea (Camellia sinensis), native to China, is a semi-fermentation with a good pleasant, fragrant and sweat taste. The aroma compounds, collected by accelerated solvent extraction (ASE), compared to distillation extraction (SDE), were investigated. The major results are as fellows: Using ASE, 63 kinds of aroma compounds of Fenghuangdancong Oolong tea cvs. Guihua were identified, whereas 55 aroma compounds by SDE, respectively. The major aroma compounds of Oolong tea collected by ASE were linalool, nerolidol, nonanoic acid, decanoic acid, geraniol and 3,7-dimethyl-1,5,7-octatriene-3-ol. ASE exhibited advantages in collecting actual aroma compounds, modifying the analysis of tea heat-instable aroma compounds and maintaining a good analytic stability.