乙酰化丝素蛋白基表面活性剂的合成

研究乙酰化丝素蛋白基表面活性剂合成的工艺条件，主要对丝素蛋白乙酰化工艺参数，如丝素蛋白质量分数、乙酸酐用量、反应时间、反应温度、反应pH值等影响因素进行探讨，用正交试验确定最佳的酰化工艺参数。乙酰化的最佳工艺条件：丝素蛋白质量分数2%、乙酸酐用量15%、反应时间20min、反应温度40℃、反应pH值为8.5，乙酰化程度达到47.8%。在最佳工艺条件下，经过减压蒸馏、喷雾干燥处理得到乙酰化丝素蛋白基表面活性剂，产率达到60.9%。通过红外光谱和1HNMR对产品进行结构表征。测定产品的表面活性，考察其生物降解性能，在10d内降解度为98.9%，属于易降解表面活性剂。

Synthesis of Acetylated Silk Protein Surfactant

The acetylation process of silk protein for preparing a green and nutrition surfactant was investigated in this study. Some major technological parameters such as silk protein concentration, acetic anhydride amount, reaction temperature, reaction time, and pH were explored using orthogonal array design. The optimal values of the parameters were silk protein concentration 2%, acetic anhydride amount 15%, pH 8.5 and temperature 40 ℃ for a reaction duration of 20 min. Under these optimal conditions, the acetylation rate reached 47.8%. Acetylated silk protein surfactant was obtained through vacuum distillation and spray-drying. The structure of the product obtained was characterized by UV, IR and 1H-NMR spectra. After 10 days of storage, the biodegradability of acetylated silk protein surfactant was 98.9%. Therefore, the surfactant can be recognized as a biodegradable surfactant.