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| 大叶麻竹笋多糖分离纯化工艺 | |
| 引用本文： | 吴金松,郑炯,夏雪娟,汪健,阚建全. 大叶麻竹笋多糖分离纯化工艺[J]. 食品科学, 2015, 36(2): 80-84. DOI: 10.7506/spkx1002-6630-201502015 |
| 作者姓名： | 吴金松 郑炯 夏雪娟 汪健 阚建全 |
| 作者单位： | 1.西南大学食品科学学院，重庆 400715；2.郑州科技学院食品科学与工程学院，河南 郑州 450064；3.重庆市食品药品监督检验所，重庆 401121 |
| 摘 要： | 以水提醇沉法提取到的大叶麻竹笋粗多糖为原料，对其进行脱蛋白、透析脱色、DEAE-52纤维素柱层析分级、Sephadex-50葡聚糖凝胶柱分级处理，探究大叶麻竹笋多糖的分离纯化工艺。研究结果表明，木瓜蛋白酶结合Sevag法是最佳脱蛋白条件；进行DEAE-52纤维素柱分级，以纯水、0.05 mol/L和0.1 mol/L NaCl溶液洗脱获得了3 种主要的大叶麻竹笋多糖组分BSP1、BSP2、BSP3；再进行Sephadex-50葡聚糖凝胶分级，分别纯化得到了BSP1A、BSP2A、BSP3B 3 种多糖组分，这3 种多糖组分基本不含蛋白质和核酸，且纯度均达到了95.3%以上。 |
| 关 键 词： | 大叶麻竹笋多糖 脱蛋白法 透析脱色法 分级纯化法 纯度鉴定 |
Separation and Purification of Polysaccharides from Ma Bamboo Shoots (Dendrocalamus latiflorus) |
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| WU Jinsong;ZHENG Jiong;XIA Xuejuan;WANG Jian;KAN Jianquan. Separation and Purification of Polysaccharides from Ma Bamboo Shoots (Dendrocalamus latiflorus)[J]. Food Science, 2015, 36(2): 80-84. DOI: 10.7506/spkx1002-6630-201502015 | |
| Authors: | WU Jinsong ZHENG Jiong XIA Xuejuan WANG Jian KAN Jianquan |
| Affiliation: | 1. College of Food Science, Southwest University, Chongqing 400715, China; 2. College of Food Science and Engineering,ZhengZhou University of Science and Technology, Zhengzhou 450064, China;3. Chongqing Institute for Food and Drug Control, Chongqing 401121, China |
| Abstract: | The aim of this study was to optimize the separation and purification of polysaccharides from Ma bamboo shoots (Dendrocalamus latiflorus). Crude polysaccharides were extracted from bamboo shoots by hot water extraction method, deproteinized, decolorized by dialysis and fractionated by DEAE-52 cellulose and Sephadex-50 column chromatography. The results showed that papain treatment combined with Sevag method was the best deproteinization condition. Three major polysaccharide fractions were eluted from DEAE-52 cellulose with water, 0.05 mol/L NaCl and 0.1 mol/L NaCl, namely BSP1, BSP2 and BSP3, respectively. The fractions were further purified by Sephadex-50 column chromatography to produce BSP1A, BSP2A and BSP3B, respectively. These further purified polysaccharides were found to contain almost no protein or nucleic acid with a purity more than 95.3%. |
| Keywords: | bamboo shoot polysaccharide deproteinization dialysis fractionation purity identification |
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