| 火龙果皮原花青素提取纯化及定性分析 |
| |
| 引用本文： | 杨志娟,曾真,吴晓萍.火龙果皮原花青素提取纯化及定性分析[J].食品科学,2015,36(2):75-79. |
| |
| 作者姓名： | 杨志娟 曾真 吴晓萍 |
| |
| 作者单位： | 广东海洋大学食品科技学院，广东 湛江 524025 |
| |
| 摘 要： | 采用大孔树脂对原花青素粗品进行分离纯化。比较了AB-8、DM130、ADS-17三种大孔树脂对原花青素的吸附和解吸能力,选择AB-8型大孔树脂为吸附树脂。对AB-8大孔树脂吸附解吸条件进行优化,探讨上样流速、解吸流速和解吸剂体积分数3个因素对分离纯化效果的影响。结果表明:AB-8大孔树脂提纯工艺的最佳条件为上样流速2 m L/min、解吸流速1 m L/min、解吸剂体积分数50%。通过花青素反应与高效液相色谱-质谱对提取物进行定性分析,证实该提取物中含有的原花青素为儿茶素、表儿茶素和二聚体,且原花青素纯度为96.65%。
|
| 关 键 词： | 火龙果皮 原花青素 提取纯化 定性分析 |
Extraction,Purification and Qualitative Analysis of Proanthocyanidins from Pitaya Peel |
| |
| YANG Zhijuan;ZENG Zhen;WU Xiaoping.Extraction,Purification and Qualitative Analysis of Proanthocyanidins from Pitaya Peel[J].Food Science,2015,36(2):75-79. |
| |
| Authors: | YANG Zhijuan;ZENG Zhen;WU Xiaoping |
| |
| Affiliation: | College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524025, China |
| |
| Abstract: | The crude proanthocyanidins extracted from pitaya peel were purified by macroporous adsorption resin. AB-8
macroporous resin was selected as the best adsorbent for proanthocyanidins based on adsorption and desorption capacities in
comparison with DM130 and ADS-17. Effects of sample loading flow rate, elution flow rate and eluent concentration on the
purification of pitaya peel proanthocyanidins by AB-8 macroporous resin were investigated. The best results were achieved
by sample loading at a flow rate of 2 mL/min followed by elution with 50% ethanol at a flow rate of 1 mL/min. The purified
product contained 96.65% proanthocyanidins including catechin, epicatechin and dimmer as indicated by colorimetric
observation and qualitative analysis by high performance liquid chromatography-electrospray ionization-mass spectrometry
(HPLC-ESI-MS). |
| |
| Keywords: | pitaya peel proanthocyanidins extraction and purification qualitative analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
