动态超高压微射流辅助提取对滇黄精多糖结构和活性的影响

为评价动态超高压微射流技术对滇黄精多糖提取的适用性，通过比较动态超高压微射流辅助提取与常规热水浸提所得滇黄精多糖的得率、结构特征（分子质量、黏度、单糖组成、红外光谱）、功能活性（1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基清除能力、α-糖苷酶抑制活性和酪氨酸酶抑制活性），明确动态超高压微射流提取对滇黄精多糖结构和活性的影响。结果表明，动态超高压微射流辅助提取滇黄精多糖得率为热水浸提得率的1.63 倍；与热水浸提相比，动态超高压微射流提取技术降低了滇黄精多糖的分子质量（27.90%）和黏度（29.04%），改变了滇黄精多糖的单糖组成，提高了滇黄精多糖的DPPH自由基清除能力和α-糖苷酶抑制活性，但对滇黄精多糖主要结构和酪氨酸酶抑制活性无明显影响。综上，动态超高压微射流技术在提高滇黄精多糖得率、增强其抗氧化和降血糖活性方面均有明显优势，是一种具有前景的滇黄精多糖提取方法，本研究可为滇黄精多糖的高效提取提供一定的科学依据。

Effect of Dynamic High-Pressure Microfluidization Assisted Extraction on the Structure and Activity of Polysaccharides from Polygonatum kingianum Coll. et Hemsl.

In order to evaluate the applicability of dynamic high-pressure microfluidization (DHPM) for the extraction of polysaccharides from Polygonatum kingianum Coll. et Hemsl. (PKP), the effects of DHPM assisted extraction on the yield, structural characteristics (molecular mass, viscosity, monosaccharide composition, and infrared spectrum) and functional properties (1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, α-glycosidase inhibitory activity and tyrosinase inhibitory activity) of PKP were evaluated in comparison with traditional hot water extraction. The results showed that compared to traditional hot water extraction, DHPM assisted extraction led to a 1.63-fold increase in the yield of PKP, decreased its molecular mass and viscosity by 27.90% and 29.04%, respectively, changed its monosaccharide composition, and increased its DPPH radical scavenging capacity and α-glycosidase inhibitory activity, but had little effect on its main structure or tyrosinase inhibitory activity. To sum up, DHPM has obvious advantages in improving the extraction yield and antioxidant and hypoglycemic activities of PKP and therefore is a promising method for PKP extraction. This study will provide a scientific basis for the high-efficiency extraction of PKP.