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| 碎米荠硒多糖的分离纯化及光谱分析 | |
| 引用本文： | 石宝霞,车会莲,赵利霞,谭军,潘思轶,汪兴平,柳玉.碎米荠硒多糖的分离纯化及光谱分析[J].食品科学,2007,28(6):298-302. |
| 作者姓名： | 石宝霞 车会莲 赵利霞 谭军 潘思轶 汪兴平 柳玉 |
| 作者单位： | 1. 中国农业大学食品科学与营养工程学院,北京,100083 2. 北京市食品酿造研究所,北京,100050 3. 华中农业大学食品科技学院,湖北,武汉,430070 4. 湖北民族学院,湖北,恩施,445000 |
| 摘 要： | 本研究利用DEAE-Cellulose-52离子交换层析技术,采用步进式洗脱模式成功地将碎米荠硒多糖粗品进行Sephadex G-200葡聚糖凝胶柱层析,得到四种均一的硒多糖,SDS-PAGE电泳法鉴定其纯度。并采用紫外分光光度计、傅立叶变换红外光谱仪对硒多糖的结构进行了分析。 |
| 关 键 词： | 硒多糖 分离纯化 红外光谱 |
| 文章编号： | 1002-6630（2007）06-0298-05 |
| 修稿时间： | 2007-02-01 |
Study on Purification and Spectra Analysis of Cardamine Urbaniana O.E. Schlz-Selenium Polysaccharide |
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| SHI Bao-xia,CHE Hui-lian,ZHAO Li-xia,TAN Jun,PAN Si-yi,WANG Xing-ping,LIU Yu.Study on Purification and Spectra Analysis of Cardamine Urbaniana O.E. Schlz-Selenium Polysaccharide[J].Food Science,2007,28(6):298-302. | |
| Authors: | SHI Bao-xia CHE Hui-lian ZHAO Li-xia TAN Jun PAN Si-yi WANG Xing-ping LIU Yu |
| Affiliation: | 1.College of Food Science and Nutritional Engineering, Chinese Agricultural University, Beijing 100083, China; 2.Beijing Food Brewing Institute, Beijing 100050, China; 3.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 4. Hubei Institute for Nationalities, Enshi 445000, China |
| Abstract: | DEAE-Cellulose-52 ion-exchange chromatography was used to separate CUS-SeP. It was successful to obtain four fractions, by the step gradient elution. PE was further purified by SephdexG-200. Electrophoresis was applied to identify the purity of PE, and identified to be electrophoresis grade purity. The structure characteristics of CUS-SeP were analysed with UV and FTIR. |
| Keywords: | selenium polysaccharide purification spectra analysis |
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