| 促进纳他霉素合成的真菌诱导子筛选及诱导条件优化 |
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| 引用本文： | 魏宝东,潘娅慧,王亚男,王利艳.促进纳他霉素合成的真菌诱导子筛选及诱导条件优化[J].食品科学,2013,34(7):196-200. |
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| 作者姓名： | 魏宝东 潘娅慧 王亚男 王利艳 |
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| 作者单位： | 沈阳农业大学食品学院，辽宁 沈阳 110866 |
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| 基金项目： | 辽宁省教育厅科研项目(L2012248) |
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| 摘 要： | 采用真菌诱导子提高纳他霉素的产量，通过初筛，获得具有正向调节作用的真菌诱导子；采用Box-Behnken试验设计建立数学模型，进行响应面分析优化，确定诱导条件。结果表明：黑曲霉代谢产物诱导子能够有效提高纳他霉素的产量；质量浓度为122μg/mL的黑曲霉代谢产物诱导子在纳他霉素发酵18.3h后加入，诱导78.5h时，发酵液中纳他霉素的发酵产量可达到2.89g/L，为空白对照组的1.68倍。
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| 关 键 词： | 真菌诱导子 发酵 纳他霉素 响应面 |
| 收稿时间： | 2012-05-10 |
Screening of Fungal Elicitor for Fermentative Production of Natamycin and Optimization of Induction Conditions |
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| WEI Bao-dong,PAN Ya-hui,WANG Ya-nan,WANG Li-yan.Screening of Fungal Elicitor for Fermentative Production of Natamycin and Optimization of Induction Conditions[J].Food Science,2013,34(7):196-200. |
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| Authors: | WEI Bao-dong PAN Ya-hui WANG Ya-nan WANG Li-yan |
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| Affiliation: | College of Food Science, Shenyang Agricultural University, Shenyang 110866, China |
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| Abstract: | Preliminary screening was carried out to obtain fungal elicitor that induces natamycin production by
Streptomyces chattanovgensis. The optimal induction conditions were established by mathematical modelling mathematical
modelling based on Box-Behnken experimental design and response surface analysis. The potent inducing effect of the
fungal elicitors on natamycin production was confirmed. When fungal elicitor from Aspergillus niger was added to a final
concentration of 122 μg/mL after 18.3 h natamycin fermentation and incubated for 78.5 h, the observed average yield of
natamycin in fermentation broth was 2.89 g/L, 1.68 times higher than the blank control. |
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| Keywords: | fungal elicitor fermentation natamycin response surface analysis |
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