| 褐蘑菇水溶性多糖分级组分的抗氧化性能 |
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| 引用本文： | 李芳亮,高杨,刘莹,王锐.褐蘑菇水溶性多糖分级组分的抗氧化性能[J].食品科学,2011,32(21):37. |
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| 作者姓名： | 李芳亮 高杨 刘莹 王锐 |
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| 作者单位： | 辽宁工程技术大学理学院;辽宁省阜新市科技局; |
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| 基金项目： | 辽宁工程技术大学优秀青年科学研究基金项目(07-127) |
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| 摘 要： | 采用水提醇沉法提取并精制褐蘑菇多糖(WPPA),用体积分数60%、70%和80%乙醇分级醇沉分离得3种粗多糖WPPA60、WPPA70和WPPA80;通过还原力、清除超氧阴离子自由基(O-2.)、清除羟自由基(.OH)和抑制H2O2诱导红细胞氧化溶血实验评价3种褐蘑菇多糖体外抗氧化能力。结果表明:WPPA60、WPPA70和WPPA80具有较强的还原能力,对O-2.和.OH具有较强的清除作用;对H2O2诱导红细胞氧化溶血及MDA生成有很强的抑制作用;抗氧化能力从强到弱依次是WPPA70>WPPA80>WPPA60。
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| 关 键 词： | 还原力 褐蘑菇 多糖 抗氧化 |
Antioxidant Activity of Water-soluble Polysaccharide Fractions from Portabella Mushroom Agaricus bisporus |
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| LI Fang-liang,GAO Yang,LIU Ying,WANG Rui.Antioxidant Activity of Water-soluble Polysaccharide Fractions from Portabella Mushroom Agaricus bisporus[J].Food Science,2011,32(21):37. |
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| Authors: | LI Fang-liang GAO Yang LIU Ying WANG Rui |
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| Affiliation: | LI Fang-liang1,GAO Yang2,LIU Ying1,WANG Rui1(1.College of Science,Liaoning Technical University,Fuxin 123000,China,2.Fuxin Science and Technology Bureau,China) |
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| Abstract: | Water extraction followed by fractional ethanol precipitation was used to purify water-soluble polysaccharides from portabella mushroom Agaricus bisporus(WPPA).The polysaccharide fractions WPPA60,WPPA70 and WPPA80 were obtained using three ethanol concentrations: 60%,70% and 80%.Their antioxidant activity in vitro was assessed in terms of reducing power,scavenging activity against superoxide anion hydroxyl free radicals and inhibitory activity against H2O2-induced erythrocyte hemolysis.The results showed th... |
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| Keywords: | reducing power portabella mushroom Agaricus bisporus polysaccharide antioxidation |
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