| 方格星虫多糖的分离纯化及单糖组成 |
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| 引用本文： | 董兰芳,张琴,许明珠.方格星虫多糖的分离纯化及单糖组成[J].食品科学,2015,36(1):109-112. |
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| 作者姓名： | 董兰芳 张琴 许明珠 |
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| 作者单位： | 广西壮族自治区海洋研究所，广西海洋生物技术重点实验室，广西 北海 536000 |
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| 基金项目： | 广西科学研究与技术开发计划项目(桂科攻11107011-6);广西科学院基本科研业务费资助项目(12YJ25HYS20) |
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| 摘 要： | 方格星虫多糖经碱法提取、三氯乙酸脱蛋白、DEAE-52纤维素柱层析、Sephadex G-100凝胶柱层析纯化得到精制方格星虫多糖（Sipunculus nudus polysaccharide，SNPS）。测定SNPS的理化性质和单糖组成，结果表明：SNPS为白色粉末状单一组分；经理化性质测定和紫外光谱分析，表明SNPS的多糖质量分数为99.35%，糖醛酸质量分数为22.21%，不含蛋白质和核酸，不含硫酸根；气相色谱分析结果表明方格星虫多糖SNPS主要由阿拉伯糖、葡萄糖和半乳糖组成，其物质的量比为1.16∶9.54∶1。本研究表明SNPS是从方格星虫中得到的均一组分纯多糖。
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| 关 键 词： | 方格星虫 多糖 纤维素 凝胶 单糖 |
Isolation,Purification and Monosaccharide Analysis of Polysaccharide from Sipunculus nudus |
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| DONG Lanfang;ZHANG Qin;XU Mingzhu.Isolation,Purification and Monosaccharide Analysis of Polysaccharide from Sipunculus nudus[J].Food Science,2015,36(1):109-112. |
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| Authors: | DONG Lanfang;ZHANG Qin;XU Mingzhu |
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| Affiliation: | Key Laboratory of Marine Biotechnology of Guangxi, Guangxi Institute of Oceanology, Beihai 536000, China |
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| Abstract: | Crude polysaccharide was obtained from Sipunculus nudus by alkali extraction and deproteinized by Sevag
method. The polysaccharide was sequentially purified by ion exchange chromatography on DEAE-52 cellulose column
and Sephadex G-100 Gel column. Then, the purified polysaccharide from Sipunculus nudus (SNPS) was characterized
for physicochemical properties and monosaccharide composition. The results showed that SNPS with a purity of 99.35%
contained 22.21% alduronic acid without protein, nuclein or sulfate ions. Gas chromatography analysis showed that the
monosaccharide compositions of SNPS were arabinose, glucose and galactose, with a molar ratio of 1.16:9.54:1. This study
demonstrated that SNPS was a homogeneous component obtained from Sipunculus nudus. |
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| Keywords: | Sipunculus nudus polysaccharide cellulose gel monosacharide |
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