单增李斯特菌在生食鱼片中生长模型的建立

为研究生食鱼片中单增李斯特菌的生长规律,将单增李斯特菌接种到经冷杀菌后的3种生食鱼片(三文鱼片、金枪鱼片、鲷鱼片)中,分别置于4、8、15、25、35℃环境下培养,间隔适当时间取出计数。用5种常用的一级模型(Gompertz模型、Baranyi模型、Logistic模型、Richards模型和MMF模型)对实验数据进行拟合,通过比较相关系数R2和均方误差(mean square error,MSE),确定最适一级模型为Gompertz模型。建立单增李斯特菌生长动力学参数(最大比生长速率μm和迟滞期λ)关于温度、pH值和水分活度的二级平方根扩展模型,并应用相关系数R2、偏差值Bf和准确值Af进行验证。结果表明,构建的二级模型能够很好地描述生食鱼片中单增李斯特菌的生长情况。

Establishment of Predictive Model for Listeria monocytogenes Growth in Raw Fish Fillets

In order to predict the growth of Listeria monocytogenes in raw fish fillets, salmon slices, tuna slices and tilapia
slices were disinfected by cold sterilization, inoculated with Listeria monocytogenes, transferred for storage at 4, 8, 15, 25
and 35 ℃, and then counted at appropriate time intervals. Five common primary models (Gompertz, Baranyi, Logistic,
Richards and MMF) were applied to fit the growth curve for Listeria monocytogenes. The correlation coefficient (R2) and
standard deviation (MSE) were compared to identify Gompertz model as the best fitting model. Extended square-root
models concerning growth kinetic parameters (maximum specific growth rate and lag period) of Listeria monocytogenes
as a function of temperature, pH, water activity (aw) and were developed. The R2, bias value (Bf) and accuracy (Af) were
calculated to evaluate the established models. The results showed that the secondary models could describe the growth of
Listeria monocytogenes in raw fish fillets with satisfaction.