应用Illumina高通量测序技术探究长根菇多糖对小鼠肠道菌群的影响

本研究采用Illumina高通量测序技术检测长根菇多糖对昆明小鼠肠道菌群的影响。利用盐酸林可霉素灌胃建立肠道微生态失调小鼠模型。将60只小鼠随机分为5组:空白对照组、自然恢复组、长根菇多糖低剂量组、长根菇多糖中剂量组和长根菇多糖高剂量组。无菌条件下采集小鼠盲肠内容物,运用Illumina高通量测序技术分析各组小鼠盲肠内容物中菌群的结构。结果表明:空白对照组小鼠肠道菌群物种多样性最高,长根菇多糖各剂量组次之,自然恢复组最低;另外,各组小鼠肠道有益菌与致病菌比例差异显著,长根菇多糖各剂量组有益菌群相对比例高于自然恢复组,而自然恢复组小鼠肠道中致病菌比例最高。长根菇多糖对小鼠的肠道菌群失调具有恢复和改善作用。

Effect of Oudemansiella radicate Polysaccharide on Intestinal Microbiota in Mice Evaluated by Illumina High-Throughput Sequencing

The effect of Oudemansiella radicate polysaccharide (ORP) on the intestinal microbiota in Kunming male mice
was studied by Illumina high-throughput sequencing in this investigation. The mouse model of intestinal dysbiosis was
established by intragastric administration of lincomycin hydrochloride. Sixty mice were randomly divided into five groups
including normal control group, natural recovery group and three groups treated with Oudemansiella radicate polysaccharide
at different doses. Cecal contents were collected aseptically, and the intestinal microbiota was detected by illumina highthroughput
sequencing. The results showed that the diversity and abundance of intestinal bacteria were varied in the mice
in the five groups. Among these, the normal control group had the highest intestinal microbiota diversity followed by the
ORP treatment groups, and the intestinal microbiota of the natural recovery group exhibited the lowest species diversity. In
addition, the proportions of beneficial and pathogenic bacteria in the mouse intestine differed significantly among the five
groups; the proportion of beneficial bacteria was higher in the three treatment groups than in the two other groups and the
highest proportion of pathogenic bacteria was observed in the natural recovery group. indicating that Oudemansiella radicate
polysaccharide could improve and recover the intestinal microbiota of mice with intestinal dysbiosis.