首页 | 本学科首页   官方微博 | 高级检索  
     

核酸适配体技术在食品重金属检测中的应用研究进展
引用本文:于寒松,隋佳辰,代佳宇,宋战昀,郑 言,杨 倩,王向辉,张 健,李敏思.核酸适配体技术在食品重金属检测中的应用研究进展[J].食品科学,2015,36(15):228-233.
作者姓名:于寒松  隋佳辰  代佳宇  宋战昀  郑 言  杨 倩  王向辉  张 健  李敏思
作者单位:1.吉林农业大学食品科学与工程学院,吉林 长春 130118;2.吉林出入境检验检疫局,吉林 长春 130062;; 3.甘肃农业大学动物医学院,甘肃 兰州 730070
摘    要:近年来食品中重金属超标对人们健康构成了威胁,所以对食品中重金属检测技术的开发成为了重要研究内容。虽然传统的检测技术对重金属进行了高选择性和高灵敏度的检测,但是仍需要一种简单、快速、有效、成本低廉的方法用于食品安全领域的快速检测。核酸适配体为一段单链DNA或者RNA序列,是经体外筛选技术筛选出的能特异结合蛋白质或其他小分子物质的寡聚核苷酸片段。核酸适配体具有靶分子广、灵敏度高、特异性强、稳定性好等特点,因而被广泛用于食品安全检测领域。本文综述近年来核酸适配体技术对食品中Hg2+、As3+、Pb2+的检测,并对核酸适体技术在食品重金属检测领域的应用前景进行了展望。

关 键 词:重金属  核酸适配体  配体指数级富集系统进化技术  检测  

Advances in the Application of Aptamers to Detect Heavy Metals in Foods
YU Hansong,SUI Jiachen,DAI Jiayu,SONG Zhanyun,ZHENG Yan,YANG Qian,WANG Xianghui,ZHANG Jian,LI Minsi.Advances in the Application of Aptamers to Detect Heavy Metals in Foods[J].Food Science,2015,36(15):228-233.
Authors:YU Hansong  SUI Jiachen  DAI Jiayu  SONG Zhanyun  ZHENG Yan  YANG Qian  WANG Xianghui  ZHANG Jian  LI Minsi
Affiliation:1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China; 2. Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun 130062, China; 3. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China
Abstract:In recent years, excessive heavy metals in foods pose a threat to human health, so the development of techniques
to detect heavy metals in foods has become an important research task. Although traditional techniques for heavy metal
detection have high selectivity and high sensitivity, a simple, fast, effective and inexpensive method for rapid detection of
food safety is still highly desired. Aptamers (aptamer) is a piece of single-stranded DNA or RNA sequence or oligonucleotide
fragments obtained by in vitro selection (SELEX, systematic evolution of ligands by exponential enrichment), which is
specifically bound to protein or smaller molecules. As a target molecule with high sensitivity, high specificity and good
stability, in recent years, it has been widely used in the field of food safety detection. This article reviews the progress made
in recent years in applying aptamer technology for the detection of heavy metals such as Hg2+, As3+ and Pb2+ in foods and
future prospects are discussed.
Keywords:heavy metal  aptamer  systematic evolution of ligands by exponential enrichment  detection  
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《食品科学》浏览原始摘要信息
点击此处可从《食品科学》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号