响应面试验优化新疆阿魏根多糖微波辅助提取工艺及其体外抗氧化活性

为了获得微波提取新疆阿魏根多糖的最佳工艺,以及新疆阿魏粗多糖的体外抗氧化活性,采用响应面法优化新疆阿魏水溶性多糖的微波提取工艺,在单因素试验的基础上选取液料比、提取温度、提取时间、微波功率进行试验设计,以所得多糖质量与苯酚硫酸法测得的多糖含量百分数的乘积作为优化指标,并检测新疆阿魏根多糖体外清除1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基的活性。结果:最优工艺条件为液料比120∶1(m L/g)、提取时间13 min、提取温度80℃、微波功率600 W,多糖实际得率为6.93%,接近于理论值。新疆阿魏根多糖对DPPH自由基有很好的清除作用,当质量浓度为1 000μg/m L时,新疆阿魏根多糖对DPPH自由基的清除率为91.67%,作用接近于VC的清除作用。

Extraction Optimization of Polysaccharides from the Roots of Ferula sinkiangensis K.M.Shen and Their Antioxidant Activities in Vitro

This study aimed to obtain the optimum microwave-assisted extraction process for polysaccharides from the
roots of Ferula sinkiangensis K. M. Shen and to examine the antioxidant activity of the crude polysaccharides in vitro. The
response surface methodology (RSM) was used to optimize the extraction process. Liquid/solid ratio, extraction temperature,
extraction time, microwave power were selected as independent variables on the basis of single factor experiments. The yield
of purified polysaccharides was chosen as the response variable. The optimum conditions for microwave-assisted extraction
were determined as follows: liquid/solid ratio, 120:1 (mL/g); extraction temperature, 80 ℃; extraction time, 13 min; and
microwave power, 600 W. Experiments done under these conditions resulted in an extraction yield of 6.93%, agreeing
with the predicted value. The crude polysaccharides extracted from the roots of Ferula sinkiangensis K. M. had a potent
scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals, and could scavenge 91.67% of the free radicals at
1 000 μg/mL, which was close to that of VC.