邻氨基苯酚衍生气相色谱-质谱分析不饱和脂肪酸

以邻氨基苯酚为脂肪酸的衍生化试剂，柱前衍生气相色谱-EI质谱分析不饱和脂肪酸，邻氨基苯酚将不饱和脂肪酸羧基化学修饰成含氮杂环，从而避免了链烯基中碳碳双键在EI源中的移动，并使质谱呈现显示双键位置的规范信息。通过解析不饱和脂肪酸邻氨基苯酚衍生产物的EI质谱图，得到了不饱和脂肪酸中碳碳双键的定位规则，从而为分析不饱和脂肪酸精细结构提供了手段。用脂肪酸邻氨基苯酚衍生气相色谱-EI质谱法分析了葵子油脂肪酸，鉴定出2种不饱和脂肪酸，确定了脂肪酸中碳碳双键的位置，结果与文献一致。

Analysis of Unsaturated Fatty Acids by Gas Chromatography-Mass Spectrometry with Ortho-Aminophenol Pre-Column Derivatization

A gas chromatography-electron impact mass spectrometric(GC-EIMS) method was developed for analyzing the unsaturated fatty acids, while ortho-aminophenol was used for the pre-column derivatization reagent of unsaturated fatty acids so as to modify carboxy1 group into nitrogen-containing heterocycle which effectively suppressed the migration of double bonds of the alkeny1 under the electron impact source and led to regular informative mass spectra regarding the position of the olefinic bonds in the chain .The empirical rule in which the position of double bonds of olefinic acids was located was refered to by analyzing electron impact spectra of the derivatives of the fatty acids modified by ortho-aminophenol reagent. This methodprovides a new technical tool for analyzing the fine structure of unsaturated fatty acids. Ortho-aminophenol is used as derivatization reagent to the fatty acids and 2 unsaturated fatty acids in sunflower oil are identified according to the analysis of GC-EIMS .The position of double bonds of the unsaturated fatty acids from sunflower oil is located and the results are in good agreement with bibliography.