响应面法优化超声波辅助提取仿栗籽油工艺及其脂肪酸组成分析

采用超声波辅助提取仿栗籽油,通过单因素试验和响应面法(RSM)对提取工艺进行优化,并利用气相色谱-质谱联用法测定仿栗籽油的脂肪酸组成。结果表明,超声波辅助提取仿栗籽油的优化工艺条件为以环己烷为提取溶剂、超声工作/间歇时间为3s/1s、超声功率540W、超声时间18min、提取温度60℃、液料比8.6:1(g/mL),在此工艺条件下,仿栗籽油提取率可达94.53%。气相色谱-质谱联用测定结果表明仿栗籽油中富含不饱和脂肪酸,总含量达到70.13%,其中油酸和亚油酸的含量分别为53.95%、16.18%。

Response Surface Optimization of Ultrasonic-Assisted Extraction and Fatty Acid Composition Analysis of Sloanea hemsleyana Seed Oil

Response surface methodology(RSM) was employed to optimize the ultrasound-assisted extraction of essential oil from Sloanea hemsleyana seeds.Meanwhile,the chemical composition of the extracted essential oil was analyzed by GC-MS.The results showed that the optimal extraction conditions were cyclohexane as the best extraction solvent,single ultrasonic treatment for 3 s at a power level of 540 W followed by a 1-s interval,18 min of total extraction time,and 1:8.6 of solid-to-liquid ratio,resulting in an oil yield of 94.53%.The essential oil obtained under these conditions was abundant in unsaturated fatty acids with a total content of 70.13%.The contents of oleic acid and linoleic acid were 53.95% and 16.18%,respectively.