| 基于柠檬酸-铕纳米配位聚合物构建荧光探针快速检测汤煲中5’-肌苷酸含量 |
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| 引用本文： | 高田毅,孙冲,朱宏星,黄杨,曹锦轩,王道营.基于柠檬酸-铕纳米配位聚合物构建荧光探针快速检测汤煲中5’-肌苷酸含量[J].食品科学,2021,42(24):283-288. |
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| 作者姓名： | 高田毅 孙冲 朱宏星 黄杨 曹锦轩 王道营 |
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| 作者单位： | （1.宁波大学食品与药学学院，浙江省动物蛋白食品精深加工技术重点实验室，浙江 宁波 315800；2.江苏省农业科学院农产品加工研究所，江苏 南京 210014） |
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| 基金项目： | 国家自然科学基金面上项目（31972137）；
省部共建国家重点实验室培育基地-江苏省食品质量安全重点实验室项目（2019JG001） |
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| 摘 要： | 基于柠檬酸-铕金属有机纳米配体聚合物（citrate/europium lanthanide coordination polymer nanoparticles，Cit/Eu LCP NPs）构建快速检测肉品汤煲中5’-肌苷酸（inosine-5’-monophosphate，5’-IMP）的荧光探针。研究结果表明，5’-IMP对Cit/Eu?LCP?NPs有良好的荧光猝灭作用。在最佳条件下，该荧光探针在5’-IMP?2.5～200?μg/mL的质量浓度范围内呈现出良好的线性关系，检出限为0.17?μg/mL，且具备良好的抗干扰、稳定性和重复性。为了验证方法可行性，将该方法应用于实际鸡汤样品中的5’-IMP检测，测得加标回收率为97.85%～103.95%，可为快速检测肉品汤煲中5’-IMP提供新的思路和方法。
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| 关 键 词： | 镧系金属 配位聚合物 荧光探针 检测分析 |
Rapid Detection of Inosine-5’-monophosphate in Soup Based on Citrate/Eeuropium Nano-coordination Polymer as a Fluorescence Probe |
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| GAO Tianyi,SUN Chong,ZHU Hongxing,HUANG Yang,CAO Jinxuan,WANG Daoying.Rapid Detection of Inosine-5’-monophosphate in Soup Based on Citrate/Eeuropium Nano-coordination Polymer as a Fluorescence Probe[J].Food Science,2021,42(24):283-288. |
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| Authors: | GAO Tianyi SUN Chong ZHU Hongxing HUANG Yang CAO Jinxuan WANG Daoying |
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| Affiliation: | (1. Key Laboratory of Food Deep Processing Technology of Animal Protein of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China; 2. Institute of Agri-products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China) |
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| Abstract: | In this study, a fluorescent probe based on citrate/europium lanthanide coordination polymer nanoparticles (Cit/Eu LCP NPs) was constructed to detect inosine-5’-monophosphate (5’-IMP) in soup. The results showed that 5’-IMP could quench the fluorescence of Cit/Eu LCP NPs. Under the optimal conditions, the fluorescence probe showed a good linear relationship with 5’-IMP in the concentration range of 2.5–200 μg/mL with a detection limit of 0.17 μg/mL. Meanwhile, the fluorescent probe had good anti-interference ability, stability and repeatability. In order to verify its feasibility, Cit/Eu LCP NPs was applied to the determination of 5’-IMP in a real chicken soup sample, indicating that the recoveries of the added 5’-IMP was 97.85%–103.95%. Therefore, the Cit/Eu LCP NPs-based fluorescent probe can be used for the rapid detection of 5’-IMP in soup. |
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| Keywords: | lanthanide metals coordination polymer fluorescence probe detection and analysis |
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