UPLC-MS/MS快速筛查豆芽中27 种植物生长调节剂和抗生素类药物

目的：建立超高效液相色谱-串联质谱快速筛查豆芽中27 种植物生长调节剂和抗生素类药物的方法。方法：采用Agilent Eclipse Plus-C18（2.1 mm×100 mm，1.8 μm）色谱柱，以0.01%甲酸为流动相A，甲醇为流动相B，梯度洗脱，流速为0.4 mL/min，质谱选择多反应监测模式对27 种植物生长调节剂和抗生素类药物进行定性和定量检测。结果：该方法线性范围宽、相关性好，R2≥0.998 5；精密度相对标准偏差为1.1%～3.5%（n=6）；方法采用3 个添加水平进行加样回收实验，回收率范围为80.5%～114.9%；检出限为0.5～50 μg/kg，定量限为1.5～150 μg/kg。结论：该方法快速、专属性强、灵敏度高，可同时完成豆芽中27 种植物生长调节剂和抗生素类药物的检测。

Rapid Screening of 27 Plant Growth Regulator and Antibiotic Residues in Bean Sprouts by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

Objective: An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for rapid screening of 27 plant growth regulator and antibiotic residues in bean sprouts. Methods: The analytes were separated on an Agilent Eclipse Plus-C18 chromatographic column (2.1 mm × 100 mm, 1.8 μm) using gradient elution with 0.01% formic acid as mobile phase A and methanol as mobile phase B at a flow rate of 0.4 mL/min. Qualitative and quantitative analysis were conducted by tandem mass spectrometry in a multiple reaction monitoring (MRM) mode. Results: The method had a wide linear range and good linearity, with correlation coefficient (R2) ≥ 0.998 5, and the precision expressed as relative standard deviation (RSD) ranged from 1.1% to 3.5% (n = 6). The recoveries at three spiked concentrations ranged from 80.5% to 114.9%. The limits of detection (LOQ) and limits of quantitation (LOQ) were 0.5–50 and 1.5–150 μg/kg, respectively. Conclusion: This method is rapid, accurate and highly sensitive, and can be used for simultaneous detection of the 27 plant growth regulator and antibiotic residues in bean sprouts.