玉米须黄酮类测定方法的研究

本文选用多种黄酮类测定方法，通过对玉米须黄酮类提取液、单体及芦丁标样用不同的显色剂反应后，测定各反应液在200～600nm范围内的吸收曲线，确定了适合玉米须黄酮类定量测定的波长，并对不同测定方法的定量结果进行了比较。结果表明，Al（NO3)3)，比色法不适合测定玉米须黄酮类的含量，而AlCl3比色法是一种简便、准确的方法，以芦丁为标样，可用于玉米须黄酮类含量的测定。

Study on Determination Methods of Flavonoids from Corn Silk

Several methods were selected to determine the content of flavonoids in corn silk, while satisfying wave length wasdecided by means of UV-visible photometer curves of flavonoids and its monomer in corn silk, as well as rutin at the range of200~600nm after reacting with different regents , and comparison of experimental results were carried out. The results showedthat Al(NO3)3 colorimetry is not suitable to determine content of flavonoids in corn silk. While AlCl3 colorimetry is a simple,accuratemethod which can be used to determine content of corn silk flaovonoids with rutin as reference substance.