两种乳酸杆菌超声破碎提取亚油酸异构酶条件的研究

本文利用超声波对两种乳酸杆菌细胞进行破碎,以提取细胞中的亚油酸异构酶,并就超声波破碎条件对亚油酸异构酶释放的影响进行了研究。通过单因素试验和正交试验得出嗜酸乳杆菌的最佳超声破碎条件为:间歇破碎次数80次(每次破碎3s,中间间隔4s),破碎菌液体积50ml,菌悬液浓度20g/60ml,破碎时的抽提缓冲液最佳pH值4.0,NaCl浓度0.2mol/L;保加利亚乳杆菌的最佳超声破碎条件为:间歇破碎次数80次(每次破碎3s,中间间隔4s),破碎菌液体积60ml,菌悬液浓度15g/50ml,破碎时的抽提缓冲液最佳pH值4.8,NaCl浓度0.3mol/L。

Research on Conditions of Ultrasonic Wave Disruption of Two Lactobacillus sp. Cells for Extraction of Linoleate Isomerase

Ultrasonic wave was used in this paper to disrupt the cells of two Lactobacillus species so as to extract linoleate isomerase from the cells, and the effects of the disruption conditions to release linoleate isomerase were studied. After single-factor experiment and orthogonal experiment, the optimum disruption conditions for Lactobacillus acidophilus were: intermittent ultrasonic treatment 80 times (time for each treatment was 3s, with a 4s interval), volume of the treated cell suspension 50ml, concentration of cell suspension 20g/60ml, pH value of the extraction buffer 4.0, and concentration of NaCl 2.0mol/L; while the optimm conditions for Lactobacillus bulgaricus were: intermittent ultrasonic treatment 80 times (time for each treatment was 3s, with a 4s interval), volume of the treated cell suspension 60ml, concentration of cell suspension 15g/50ml, pH value of the extraction buffer 4.8, and concentration of NaCl 3.0mol/L.