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质谱法测定4种真菌毒素与载体蛋白偶联物的结合比
引用本文:王莹,王希春,金福源,何成华,郑浩,张海彬. 质谱法测定4种真菌毒素与载体蛋白偶联物的结合比[J]. 食品科学, 2012, 33(2): 219-223. DOI: 10.7506/spkx1002-6630-201702048
作者姓名:王莹  王希春  金福源  何成华  郑浩  张海彬
作者单位:1.南京农业大学动物医学院 2.汾湖动物防疫站
基金项目:国家“863”计划项目(2007AA10Z429)
摘    要:采用基质辅助激光解吸-电离飞行时间质谱法(matrix-assisted laser desorption/ionization time-of-flight mass spectrometry,MALDI-TOFMS)对由3种真菌毒素伏马菌素B1(FB1)、赭曲霉毒素A(OTA)、脱氧雪腐镰刀菌烯醇(DON)与两种载体蛋白卵清白蛋白(OVA)、血清白蛋白(BSA)分别偶联制备得到的4种小分子-载体蛋白偶联物FB1-OVA、FB1-BSA、OTA-BSA、DON-OVA的偶联结合比进行测定,并与紫外吸收光谱法进行比较。基质辅助激光解吸-电离飞行时间质谱法测得偶联物FB1-OVA、FB1-BSA、OTA-BSA、DON-OVA的结合比分别为5:1、11:1、3:1、0;紫外吸收光谱法测得OTA-BSA的结合比为4.4:1,DON-OVA的结合比为3.4:1,无法测定FB1-OVA、FB1-BSA结合比。

关 键 词:基质辅助激光解吸/电离飞行时间质谱法  紫外吸收光谱法  真菌毒素  载体蛋白  偶联物  结合比  

Determination of Coupling Ratios of Four Mycotoxins-Carrier Protein Conjugates by Mass Spectrometry
WANG Ying,WANG Xi-chun,JIN Fu-yuan,HE Cheng-hua,ZHENG Hao,ZHANG Hai-bin. Determination of Coupling Ratios of Four Mycotoxins-Carrier Protein Conjugates by Mass Spectrometry[J]. Food Science, 2012, 33(2): 219-223. DOI: 10.7506/spkx1002-6630-201702048
Authors:WANG Ying  WANG Xi-chun  JIN Fu-yuan  HE Cheng-hua  ZHENG Hao  ZHANG Hai-bin
Affiliation:1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;2. Fenhu Animal Epidemic Prevention Station, Wujiang 215211, China
Abstract:Three mycotoxins including fumonisin B1(FB1),ochrotoxin A(OTA) and deoxynivalenol(DON) were coupled with two carrier proteins such as ovalbumin(OVA) and bovine serum albumin(BSA),respectively.Four small molecule-carrier protein conjugates(FB1-OVA,FB1-BSA,OTA-BSA and DON-OVA) were prepared.The coupling ratios of the conjugates were determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOFMS) and ultraviolet(UV) absorption spectroscopy,respectively.The coupling ratios of FB1-OVA,FB1-BSA,OTA-BSA and DON-OVA were 5:1,11:1,3:1 and 0,respectively as determined by MALDI-TOFMS.However,the coupling ratios of OTA-BSA and DON-OVA determined by UV absorption spectroscopy were 4.4:1 and 3.4:1,respectively.The coupling ratios of FB1-OVA and FB1-BSA could not be determined by UV absorption spectroscopy.
Keywords:matrix-assisted laser desorption/ionization time-of-flight mass spectrometry  ultraviolet absorption spectroscopy  mycotoxin  carrier protein  conjugate  coupling ratio
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