| 藤茶水溶性多糖的分离纯化及性质的研究 |
| |
| 引用本文： | 罗祖友,程超,李伟,吴谋成.藤茶水溶性多糖的分离纯化及性质的研究[J].食品科学,2007,28(1):151-154. |
| |
| 作者姓名： | 罗祖友 程超 李伟 吴谋成 |
| |
| 作者单位： | 湖北民族学院生物科学与技术学院； 华中农业大学食品科技学院 湖北恩施445000； 湖北恩施445000； 湖北武汉430070； |
| |
| 基金项目： | 湖北省自然科学基金;湖北省科技攻关项目 |
| |
| 摘 要： | 经水提、醇沉、Sevag法脱蛋白的藤茶多糖(AGP),通过DEAE-SephadexA-25和SephadexG-200柱层析,得到多糖AGP-3和AGP-4。HPLC和SephadexG-200柱层析鉴定AGP-3和AGP-4为分子量相对均一的蛋白多糖,硫酸-咔唑反应、考马斯亮蓝G-250反应均呈阳性。
|
| 关 键 词： | 藤茶 多糖 分离 纯化 性质 |
| 文章编号： | 1002-6630（2007）01-0151-04 |
| 修稿时间： | 2006-09-11 |
Isolation, Purification and Characterization of Water-soluble Polysaccharides from Ampelopsis grossedentata |
| |
| LUO Zu-you,CHENG Chao,LI Wei,WU Mou-cheng.Isolation, Purification and Characterization of Water-soluble Polysaccharides from Ampelopsis grossedentata[J].Food Science,2007,28(1):151-154. |
| |
| Authors: | LUO Zu-you CHENG Chao LI Wei WU Mou-cheng |
| |
| Affiliation: | 1.School of Biological Science and Technology, Hubei Institute for Nationalities, Enshi 445000, China; 2.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China |
| |
| Abstract: | Polysaccharide from Ampelopsis grossedentata (AGP) was obtained after being extracted with hot water, precipitated with alcohol and removed free protein with Sevag method. AGP was isolated and purified by DEAE-Sephadex A-25 and Sephadex G-200 column chromatography, two pure polysaccharides (AGP-3 and AGP-4) were gained. The results of HPLC and Sephadex G-200 column chromatography illustrated that both AGP-3 and AGP-4 were protein-bond polysaccharids with comparatively homogenous molecular weights. Also, the two polysaccharids were colored by coomassie brilliant blue G-250 and carbazole reaction. |
| |
| Keywords: | Ampelopsis grossedentata polysaccharide isolation purification characterization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
