香蕉皮多糖提取条件研究

以香蕉皮为原料,提取其中具有明显抑癌活性的物质--香蕉皮多糖,通过单因子实验结合正交试验研究了香蕉皮多糖的最优提取条件,并通过凝胶渗透色谱分析了香蕉皮多糖的分子量分布。实验结果表明:香蕉皮粗多糖的最佳提取条件为:以水为提取剂,料液比1:20,80℃加热提取4次,每次提取时间1.5h。在最佳提取条件下,香蕉皮粗多糖的得率为4.25%。经凝胶渗透色谱分析测定,所得香蕉皮多糖的数均分子量和重均分子量分别为427540和433160,分散度1.013,表明香蕉皮中所含多糖分子量分布较集中,为窄分布。

Study on Extracting Conditions of Banana Skin Polysaccharide

Banana skin polysaccharide which has obvious anticancer activity was extracted from banana skin as raw material.The optimum extracting conditions were investigated through single factor experiments combined with orthogonal test.And molecular weight distribution of banana skin polysaccharide was measured by using gel permeation chromatography(GPC).The results show that the optimum extracting conditions are:ratio of banana skin to water 1:20,and temperature 70℃ for 4 times.1.5h each time.The yield of banana skin polysaccharide is 4.25% under the optimum conditions.It is shown by GPC analysis that the number average molecular weight and the weight average molecular weight of banana skin polysaccharide obtained are 427540 and 433160 respectively.And the distribution width of molecular weight is 1.031,which indicates that the molecular weight distribution of polysaccharide in banana skin is concentrated and narrowly distributed.