| 葛渣总黄酮提取及葛根素含量测定 |
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| 引用本文： | 杨树平,韩立军,朱金卫,许瑞波,蒋迎道.葛渣总黄酮提取及葛根素含量测定[J].食品科学,2011,32(14):303. |
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| 作者姓名： | 杨树平 韩立军 朱金卫 许瑞波 蒋迎道 |
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| 作者单位： | 淮海工学院化学工程学院;淮海工学院理学院; |
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| 基金项目： | 江苏省海洋资源开发研究院科技开放基金课题(JSIMR09D02); 淮海工学院自然科学基金项目(KX10019);淮海工学院引进人才启动基金项目(KK01064) |
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| 摘 要： | 目的:优化葛渣中总黄酮提取工艺条件,测定葛渣中葛根素含量。方法:以葛渣为原料、总黄酮得率为评价指标,采用L9(34)正交试验设计,考察乙醇体积分数、提取时间、固料比、提取温度对葛渣总黄酮提取效果的影响,同时采用高效液相色谱法(HPLC法)测定葛根素的含量。结果:葛渣中总黄酮的最佳提取工艺条件为以体积分数70%乙醇溶液为溶剂、提取150min、料液比1:40(g/mL)、提取温度80℃,此条件下的总黄酮得率最高,为32.41mg/g。在该提取条件下,葛根、葛渣和葛粉中总黄酮的含量分别为46.11、32.41mg/g和11.23mg/g;葛根、葛渣和葛粉中葛根素的含量分别为40.63、21.24mg/g和4.71mg/g。结论:葛渣中含有丰富的总黄酮和葛根素,该方法可作为从葛渣中提取总黄酮和葛根素的方法。
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| 关 键 词： | 葛渣 总黄酮 葛根素 高效液相色谱法 正交试验 |
Optimization of Total Flavonoids Extraction from Radix Puerariae and HPLC Determination of Puerarin |
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| YANG Shu-ping,HAN Li-jun,ZHU Jin-wei,XU Rui-bo,JIANG Ying-dao.Optimization of Total Flavonoids Extraction from Radix Puerariae and HPLC Determination of Puerarin[J].Food Science,2011,32(14):303. |
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| Authors: | YANG Shu-ping HAN Li-jun ZHU Jin-wei XU Rui-bo JIANG Ying-dao |
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| Affiliation: | YANG Shu-ping1,HAN Li-jun2,ZHU Jin-wei1,XU Rui-bo1,JIANG Ying-dao1(1.School of Chemical Engineering,Huaihai Institute of Technology,Lianyungang 222005,China,2.School of Mathematics and Science,China) |
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| Abstract: | In this paper,orthogonal array design L9(34) was employed to optimize the extraction conditions of total flavonoids from Radix Puerariae residue left after pueraria root powder extraction.The effects of extraction temperature,ethanol concentration,extraction time and material-to-liquid ratio on extraction rate of total flavonoids were probed.Meanwhile,a HPLC method to determine puerarin content in Radix Puerariae was presented based on optimized extraction process.The optimal extraction conditions were dete... |
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| Keywords: | Radix Puerariae residue total flavonoids puerarin high performance liquid chromatography orthogo-nal array design |
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