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高效液相色谱-光电二极管阵列检测器法测定野山楂中两组分的含量
引用本文:邹盛勤,陈武. 高效液相色谱-光电二极管阵列检测器法测定野山楂中两组分的含量[J]. 食品科学, 2006, 27(11): 438-440
作者姓名:邹盛勤  陈武
作者单位:宜春学院生物工程研究所江西省天然药物活性成分研究重点实验室
基金项目:国家高技术研究发展计划(863计划)
摘    要:
【摘要】 建立高效液相色谱法测定了野山楂中乌索酸和齐墩果酸的含量,同时采用光电二极管阵列检测器检测两组分的紫外光谱。色谱柱为NucleosilC18柱(4.6mm×250mm,5μm),流动相为甲醇-水(87:13,V/V),流速0.8ml/min,检测波长210nm,柱温26℃。乌索酸在0.1244~2.488μg范围内线性关系良好(r=0.9996),平均回收率为98.1%。齐墩果酸在0.1192~2.384μg范围内线性关系良好(r=0.9999),平均回收率为101.2%。方法简便、准确、重现性好、线性范围宽,适用于测定野山楂中乌索酸和齐墩果酸的含量。

关 键 词:   野山楂   乌索酸   齐墩果酸   光电二极管阵列检测器   高效液相色谱   含量测定  
文章编号:1002-6630(2006)11-0438-03
收稿时间:2006-08-19
修稿时间:2006-08-19

Determination of Two Components in Crataegus cuneata Sieb. et Zucc. by HPLC-PAD
ZOU Sheng-qin,CHEN Wu. Determination of Two Components in Crataegus cuneata Sieb. et Zucc. by HPLC-PAD[J]. Food Science, 2006, 27(11): 438-440
Authors:ZOU Sheng-qin  CHEN Wu
Affiliation:Bioengineering Research Institute,Yichun University,Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines,Yichun 336000,China
Abstract:Adetermination method of ursolic acid and oleanolic acid in Crataegus cuneata Sieb. et Zucc. by reversed-phase high performance liquid chromatography was established and at the time UV spectrum of two components was detected by means of photodiode array detector(PAD). The chromatographic column,Nucleosil C18 (4.6mm×300mm,5μm),methanol- water (87: 13,V/V) mobile phase with 0.8ml/min flow rate,the detected wavelength (210nm),and the column temperature(26℃) were adopted. The calibration curve of ursolic acid was linear under the content of 0.1244~2.488μg,the correlation coefficient was over 0.9996.The average recovery of ursolic acid was 98.1%. The calibration curve of oleanolic acid was linear under the content of 0.1192~2.384μg,the correlation coefficient was over 0.9999. The average recovery of oleanolic acid was 101.2%. The method is simple,accurate,reproducible and has wider linear range. The method can be used to determine ursolic acid and oleanolic acid in Crataegus cuneata Sieb. et Zucc.
Keywords:Crataegus cuneata Sieb.et Zucc   ursolic acid   oleanolic acid   photodiode array detector   HPLC   content determination
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