| 莲房原花青素对酪氨酸酶活力和黑色素生物合成影响的初步研究 |
| |
| 引用本文： | 段玉清,刘睿,谢笔钧.莲房原花青素对酪氨酸酶活力和黑色素生物合成影响的初步研究[J].食品科学,2004,25(3):169-174. |
| |
| 作者姓名： | 段玉清 刘睿 谢笔钧 |
| |
| 作者单位： | 华中农业大学食品科学系天然产物化学研究室,湖北,武汉,430070 |
| |
| 基金项目： | 国家自然科学基金(30270938) |
| |
| 摘 要： | 目的：探讨莲房原花青素(LSPC)对酪氨酸酶及其黑色素生物合成的抑制机理。方法：采用酪氨酸酶多巴速率氧化法体外测定药物干预前后酪氨酸酶活性，求出酪氨酸酶抑制率，并作出相应Lineweaver-Burk曲线，推断其抑制类型。结果：莲房原花青素能显著抑制酪氨酸酶活性，其半抑制浓度IC50为1．15g/L。并能有效抑制形成黑色素的中间产物L-多巴色素向黑色素转化其半抑制浓度IC50为1．750g/L。结论 莲房原花青素可抑制酪氨酸酶活性，且属于酪氨酸酶竞争性抑制剂。
|
| 关 键 词： | 莲房原花青素 酪氨酸酶 酶活力 黑色素 生物合成 抑制机理 L-多巴色素 |
| 文章编号： | 1002-6630(2004)03-0169-06 |
Preparatory Study :Effect of LSPC on the Tyrosinase Activity and the Melanin Biosynthesis of Melanin and the Tyrosinase Activity |
| |
| DUAN Yu-Qing,LIU Riu,XIE Bi-Jun.Preparatory Study :Effect of LSPC on the Tyrosinase Activity and the Melanin Biosynthesis of Melanin and the Tyrosinase Activity[J].Food Science,2004,25(3):169-174. |
| |
| Authors: | DUAN Yu-Qing LIU Riu XIE Bi-Jun |
| |
| Abstract: | Objective: To discuss the inhibiting mechanism of LSPC on the biosynthesis. Methods: Tyrosinase inhibiting rate isdetermined and Lineweaver-Burk curve is made by determining the tyrosinase activity before and after drug(LSPC) interfer-ence oxidaut of method, on which we deduce the inhibiting types of LSPC.Results: LSPC can inhibit the tyrosinase activityremarkably with the half inhibiting concentration IC50 1.155g/L, and can effectively inhibit the transition from dopa, the medial-production when melanin is formed , to melanin with the half inhibiting concentration IC50 1.750g/L.Conclusion: LSPC can inhibitthe tyrosinase activity and it is the competitive inhibitor of tyrosinase. |
| |
| Keywords: | lotus seedpod procyanidins L-Dopa |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
