不同溶剂组成对一点红提取物抗菌性能的影响

本实验选用水、50%乙醇-水、无水乙醇三种不同组成溶剂对一点红进行提取,并采用滤纸片扩散法研究了一点红不同溶剂提取物对枯芽孢草杆菌（Bacillus subtilis）、大肠杆菌（Escherichia coli）、金黄色葡萄球菌（Staphylococcus aureus）的抗菌性能,探讨不同组成溶剂对一点红抗菌有效成分提取的影响。实验结果表明水的提取物量最多,对金黄色葡萄球菌的抗菌效果明显,但对枯草芽孢杆菌和大肠杆菌的抗菌效果不明显;50%乙醇-水提取物的提取量紧次于水,抗菌性能最强且具有抗菌广谱性;无水乙醇提取物的提取量最低,对枯草杆菌和金黄色葡萄球菌的抑菌作用较强,但对大肠杆菌的抑菌作用较弱。50%乙醇-水提取物的抗菌活性最强,提取率也较高,为最佳提取溶剂。

Effects of Different Solvents on Antibacterial Activities of Emilia sonchifolia Extracts

In order to study effects on antibacterial activities of Emilia sonchifolia extracts by different solvents, the antibacterial ingredients against Emilia sonchifolia were extracted by water, 50% alcohol-water solvent and pure alcohol, respectively, and thentheir extracts were used to inhibit the growth of Bacillus subtilis, Escherichia coli or Staphylococcus aureus in this research. The results showed that water is a good solvent for extracting more ingredients from the herb, and its extracts just only showed a good antibacterial activity agcinst Staphylococcus aureus, but didn't show a strong antibacterial activity agcinst Bacillus subtilis or Escherichia coli. 50% alcohol-water solvent is an excellent solvent for extracting more ingredients from the herb, and its extracts showed the strongest antibacterial activitly against all of the three kinds of bacterium. The pure alcohol extracts is not a good solvent for extracting more ingredients from the herb, its extracts showed a stronger antibacterial activitly agcinst Bacillus subtilis or Staphylococcus aureus, and a weak antibacterial activity agcinst Escherichia coli. It could be sure from the experimental results that 50% alcohol-water solvent is a best solvent for extracting more antibacterial ingredients from the herb.