老山芹全株及其不同部位酚类物质含量及抗氧化能力分析

比较老山芹及其不同部位(嫩叶、茎)甲醇提取物总多酚、总黄酮含量,测定5种抗氧化指标(铁还原能力、超氧阴离子自由基清除能力、羟自由基清除能力、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力、2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)(2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基清除能力),研究其体外抗氧化活性间的差异。结果表明:老山芹嫩叶总多酚含量为30.51mg/g,分别为全株植物与嫩茎的1.25、1.78倍;老山芹嫩叶总黄酮含量为28.92 mg/g,分别为全株植物与嫩茎的1.34、4.00倍。在抗氧化能力测定实验中,铁还原能力、超氧阴离子自由基清除能力、羟自由基清除能力排序均为嫩叶全株植物嫩茎对照组(VC);ABTS阳离子自由基清除能力排序为嫩叶全株植物对照组(Trolox)嫩茎;DPPH自由基清除能力排序为嫩叶嫩茎对照组(VC)全株植物。老山芹及不同部位甲醇提取物对超氧阴离子自由基、羟自由基清除能力较强,对DPPH自由基、ABTS阳离子自由基清除能力次之,铁还原能力较弱。

Polyphenolic Content and Antioxidant Capacity of Whole Plants and Different Parts of Heraclenm dissectum

In this study, the total phenolic content (TPC) and total flavonoid content (TFC) and antioxidant activities of methanol extracts from whole plants and different parts of Heraclenm dissectum were compared. In order to study the difference in antioxidant activity in vitro, total antioxidant capacity was estimated by ferric reducing antioxidant power, superoxide anion, hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and 2,2’-azino-bis (3-ehtylbenzothiazoline-6- sulfonic acid) (ABTS) radical scavenging methods. The results showed that TPC in tender leaves was 30.51 mg/g, which was 1.25 and 1.78 times higher than whole plants and tender stems, respectively. TFC in tender leaves was 28.92 mg/g, which was 1.34 and 4.00 folds increased relative to whole plants and tender stems. The ferric reducing antioxidant power and superoxide anion and hydroxyl radical scavenging capacity of different parts of H. dissectum were in the decreasing order: tender leaves > whole plants > tender stems > control group (VC). The ABTS radical scavenging capacity was ranked in a descending order as follows: tender leaves > whole plants > control group (Trolox) > tender stems. The DPPH radical scavenging capacity followed the decreasing order: tender leaves > tender stems > control group (VC) > whole plants. In summary, the extracts of H. dissectum had strong scavenging capacity against superoxide anion and hydroxyl, followed by DPPH and ABTS radicals, but weak ferric reducing ability.