基于气相色谱-质谱联用技术的不同产地藜麦中脂肪酸及小分子物质组成分析

为研究国内外不同产地藜麦的成分差异,采用气相色谱-质谱联用技术对藜麦中脂肪酸及非极性小分子物质进行测定,并结合无监督模式的主成分分析和有监督模式的正交偏最小二乘判别分析对产地与成分之间的相关性进行分析。结果表明:不同产地藜麦中的脂肪酸组成相似,主要是不饱和脂肪酸,其占总脂肪酸80%以上;脂肪酸含量差异显著,其中亚油酸含量最高,均在50%左右;小分子物质含量与产地相关性显著,D-葡萄糖、蔗糖、麦芽糖和核糖醇为差异性化合物。

Analysis of Fatty Acids and Small-Molecule Substances in Quinoa from Different Areas by Gas Chromatography-Mass Spectrometry

The difference in the chemical composition of quinoa samples from different regions of China and abroad was studied. Fatty acids and non-polar small molecules were analyzed by gas chromatography-mass spectrometry (GC-MS), and the correlation between geographical origin and composition was evaluated by principal component analysis (PCA) and orthogonal partial least squares (OPLS) analysis. The results showed that fatty acid composition was similar in quinoa samples from different regions, with unsaturated fatty acid being dominant and accounting for more than 80% of total fatty acids. However, there were significant differences in fatty acid contents. Linoleic acid was the most abundant fatty acid, which represented about 50% of total fatty acids in all samples. The contents of non-polar small molecules were significantly correlated with geographical origin, and D-glucose, sucrose, maltose, and ribitol were different among samples from different regions.