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高效液相色谱-电喷雾质谱法分析牡丹花中花色苷类化合物
引用本文:樊金玲,朱文学,沈军卫,陶冠军.高效液相色谱-电喷雾质谱法分析牡丹花中花色苷类化合物[J].食品科学,2007,28(8):367-371.
作者姓名:樊金玲  朱文学  沈军卫  陶冠军
作者单位:1. 河南科技大学食品与生物工程学院,河南,洛阳,471003
2. 江南大学分析测试中心,江苏,无锡,214036
基金项目:河南省创新人才培养工程项目;河南省洛阳市科技攻关项目
摘    要:利用高效液相色谱与二极管阵列检测器/电喷雾质谱联用技术研究了牡丹花中的花色苷类化合物,分离检测了五种花色苷,结合紫外吸收光谱和质谱信息分别鉴定为矢车菊-3,5-O-二葡萄糖苷,矢车菊-3-O-葡萄糖苷、芍药-3,5-O-二葡萄糖苷、芍药-3-O-葡萄糖苷和天竺葵-3,5-O-二葡萄糖苷;比较了六个牡丹品种的花色苷组成。

关 键 词:牡丹  花色苷类化合物  高效液相色谱-电喷雾质谱联用
文章编号:1002-6630(2007)08-0367-05
修稿时间:2007-06-05

Analysis of Anthocyanins in Tree Peony Flower by High Performance Liquid Chromatography-electrospray Ionization Mass Spectroscopy
FAN Jin-ling,ZHU Wen-xue,SHEN Jun-wei,TAO Guan-jun.Analysis of Anthocyanins in Tree Peony Flower by High Performance Liquid Chromatography-electrospray Ionization Mass Spectroscopy[J].Food Science,2007,28(8):367-371.
Authors:FAN Jin-ling  ZHU Wen-xue  SHEN Jun-wei  TAO Guan-jun
Affiliation:1.College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, China ; 2.Testing and Analysis Center, Southern Yangtze University, Wuxi 214036, China
Abstract:Tree peony (Paeonia spp.) flower extracts were analyzed by high performance liquid chromatography (HPLC) with online photodiode array detection and eletrospray ionization mass. Information on identity of the major anthocyanins in extracts was obtained from their retention characteristics, UV visible spectra and mass spectra. Five anthocyanins including pelargonidin 3,5-di-O-glucoside, peonidin 3,5-di-O-glucoside, cyanidin 3,5-di-O-glucoside, peonidin 3-O-glucoside and cyaniding 3-O-glucoside were identified. The relative quantitative anthocyanin compositions of extracts of peony flowers derived from different cultivars in china were compared.
Keywords:Paeonia spp  tree peony anthocyanins  high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC/ESI-MASS)
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