| 超声波酸性丙酮法制备氯化血红素 |
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| 引用本文： | 汪长国,戴亚,刘少民,杨文敏.超声波酸性丙酮法制备氯化血红素[J].食品科学,2010,31(6):109-113. |
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| 作者姓名： | 汪长国 戴亚 刘少民 杨文敏 |
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| 作者单位： | 1.川渝中烟工业公司技术研发中心 2.中国科学技术大学化学系 |
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| 摘 要： | 研究一种利用猪血制备氯化血红素的新工艺，将超声波细胞破碎应用于酸性丙酮- 乙酸钠法制备氯化血红素。利用红外光谱法和紫外- 可见光谱法对氯化血红素产品进行表征，紫外光谱法进行氯化血红素产品含量测定。结果表明：超声波细胞破碎的最佳工艺条件为超声波功率0.8kW、破碎剂(水:乙醇，V/V)比例15:1、破碎时间6min，该法所得产品的含量为92.67%，得率为86.62%。此法可操作性强、成本低、产品纯度高。
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| 关 键 词： | 超声波 酸性丙酮法 氯化血红素 制备 正交试验 |
| 收稿时间： | 2009-05-11 |
Ultrasonic-assisted Acidic Acetone Extraction of Hemin from Porcine Blood |
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| WANG Chang-guo,DAI Ya,LIU Shao-min,YANG Wen-min.Ultrasonic-assisted Acidic Acetone Extraction of Hemin from Porcine Blood[J].Food Science,2010,31(6):109-113. |
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| Authors: | WANG Chang-guo DAI Ya LIU Shao-min YANG Wen-min |
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| Affiliation: | 1. Technical Research Center, Chuanyu Branch of China Tobacco Corporation, Chengdu 610066, China；
2. Department of Chemistry, University of Science and Technology of China, Hefei 230025, China |
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| Abstract: | To modify the acidified acetone-sodium acetate extraction technique of hemin from porcine blood,ultrasonic was used to disrupt porcine red blood cells for accelerating the release of intracellular hemoglobin.Meanwhile,extracted hemin was structurally characterized by infrared spectroscopy and UV-Visible absorption spectroscopy,and quantitative analysis was carried out by UV-Visible absorption spectroscopy.Results showed that crucial parameters affecting ultrasonic disruption of porcine red blood cells such ... |
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| Keywords: | ultrasonic acidified acetone method hemin extraction orthogonal array design |
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