无蒸煮生淀粉糖化酶霉菌的选育

从23份土样中筛选出105株野生型霉菌，其中有10株具有较高的糖化生淀粉的能力，尤以A－4（Ⅱ）、C和E6 3株菌酶活力最高，分别达112562，825．1和825．1个酶活性单位；对C株菌进行紫外光诱变，从诱变后的菌株中筛选出有较大正向突变的两株菌株C（1）和C（5），与诱变前相比，C（1）菌的生淀粉糖化酶活力提高了27．3％，经生淀粉酒精发酵试验，相同条件下酒精度提高了22．4％；C(5) 的生淀粉糖化酶活力提高了12．1％，酒精度提高了5．97％。

Selective Breeding of Moulds Produced from Raw - Starch - Saccarifying Glucoamylase without Steaming

wild strains of moulds were selected from23soil samples.Among themthere were10strains exhibiting higher sallharification effecfon s tarch.It was pointed that the enzyme activity of A -4(2),C and E6 had reached the highest activity,i.e,1125.2,825.1and825.1respectively,By inducing mutation c -strain withultraviolet ray ,two positive great mutationstrains c (1) andC(5) were obtained.Incomparisonwith th ose before inductionof mutation,th e activity of raw -starchsaccharifying enzyme (R?S?S?E)in C (1) was increasedby27.3%.In the same condition,the alcoholicdegree hadincreasedto22.4%.Bythealcoholicfermentationtest ofrawstarch,as inC (5) ,theactivityofR?S?S?Ewas increased by12.1%andalcoholic degree by5.97%.