番茄红素吸收与体内抗氧化的机理研究

番茄红素是有效的生物抗氧化剂,近年来人们对番茄红素进行了大量的研究,发现番茄红素具有多种生物学功能,如淬灭单线态氧、清除自由基、诱导细胞通讯和细胞信号、抑制肿瘤等。本实验进行了番茄红素(lycopene,LP)对小鼠抗氧化酶体系影响的研究。小鼠饲喂番茄红素4d后取血,测定血清和肝匀浆的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性、丙二醛(MDA)的含量。各番茄红素给药组的小鼠血清和肝组织的SOD、CAT和POD活性上升、MDA含量降低。与空白组比较,结果有显著性差异。番茄红素的体内抗氧化与内源性抗氧化酶体系的活性激活有关。

Study on Mechanism of Lycopene Absorption and Antioxidation Effects on Mice in vivo

It has been suggested that lycopene has many biological functions, such as quenching singlet oxygen and eliminating free radical, promotion cell communication and signal cells and inhibiting cellular malignant transformation. Since the subject of lycopene bioefficacy and bioavailability has not been studied thoroughly yet, this experiment carried out the study on lycopene affection with anti-oxidative enzymes, such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) from mice liver. Lycopene was firstly extracted from tomatoes by petroleum ether. The group of 6 mice was fed 1 week with the lycopene then killed for assay. The activities of SOD, POD, CAT and the content of malondiadehyde (MDA) were determined respectively. Due to lycopene treatment, the activities of SOD, POD increase, while the content of MDA decreases. Compared to control group, the results showed that lycopene can enhance mice's anti-oxidative capability and inhibit their lipid peroxidation.