红景天苷纳米脂质体的制备及若干工艺参数的优化

本实验首先比较了薄膜法、超声法、冻融法和反相蒸发法对红景天苷脂质体包封率的影响,结果表明红景天苷低浓度下,不同方法制得的脂质体包封率影响依次为冻融法>反相蒸发法>超声法>薄膜法,高浓度下的包封率影响不大。粒径测定表明粒子均处于纳米尺度。其次,对影响红景天苷纳米脂质体制备的反相蒸发法工艺参数进行了优化。结果表明,随着载量的降低包封率呈现上升后趋于平缓的变化。胆固醇/卵磷脂质量比1:2的嵌入量得到的脂质体包封率最高。吐温80对脂质的增溶具有十分明显的作用,2%质量分数可使得脂质体的包封率达到约80%。水合介质的离子强度对包封率的影响较大,脂质体在0.05mol/L、pH7.0的磷酸盐缓冲液体系中包封率较高。

Preparation of Salidroside Nano-liposomes and Study on Optimization of Technical Conditions

Salidroside nano-liposomes were prepared by using 4 different methods: thin film evaporation, ultrasound method, reverse phase evaporation, or freezing-thawing method. The effects of different preparation methods and salidroside concentra- tions on the formation of liposomes were examined by means of encapsulating efficiency. The experimental results showed that the encapsulating efficiency of liposomes prepared by different methods varies in the order: freezing-thawing method > reverse phase evaporation > ultrasound method > thin film evaporation. The optimization conditions of the reverse phase evaporation were as follows: the loading efficiency of salidroside reaches 40%, the ratio of cholesterol and lecithin is 1:2 (W/W) and Tween 80 added 2%, and the encapsulating efficiency 80% with pH7.0, 0.05 mol/L PBS.