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不同培养条件对银杏愈伤组织生长及黄酮含量的影响
引用本文:朱红威,邵菊芳,李庆,王丹.不同培养条件对银杏愈伤组织生长及黄酮含量的影响[J].食品科学,2007,28(11):102-105.
作者姓名:朱红威  邵菊芳  李庆  王丹
作者单位:中国矿业大学化工学院,江苏,徐州,221008
基金项目:中国矿业大学科技基金资助项目(OH060101,OH061009)
摘    要:以胚为外植体,通过固体培养,研究了培养基,激素和光照对银杏愈伤组织诱导的影响,以及继代培养中激1素和活性炭对愈伤组织生长和产黄酮的影响,结果表明:愈伤组织的诱导的最佳条件为DCR培养基 1.0mg/L2,4-D 0.5mg/LBA 完全暗条件培养。继代培养过程中既能促进愈伤组织生长又使黄酮含量最高的处理为0.3mg/LBA 2.0mg/L2,4-D,活性炭抑制愈伤组织生长但能促进黄酮的积累。

关 键 词:培养条件  银杏  愈伤组织  黄酮含量
文章编号:1002-6630(2007)11-0102-04
修稿时间:2006-09-04

Effects of Different Culture Conditions on Flavone Growth and Content of Ginkgo biloba Callus
ZHU Hong-wei,SHAO Ju-fang,LI Qing,WANG Dan.Effects of Different Culture Conditions on Flavone Growth and Content of Ginkgo biloba Callus[J].Food Science,2007,28(11):102-105.
Authors:ZHU Hong-wei  SHAO Ju-fang  LI Qing  WANG Dan
Affiliation:School of Chemical Engineering Technology ,China University of Mining Technology, Xuzhou 221008, China
Abstract:The effects of solid media, hormone and light for inducement from Ginkgo biloba embryo to callus growth were studied. The effects of hormone and carbon for callus growth and flavone output were studied as well. The results showed that: (1) The explant cultured on DCR medium with 1.0 mg/L 2,4-D 0.5 mg/L BA in dark is of the optmum conditions to induce callus growth. (2) 0.3 mg/L BA 2.0 mg/L 2,4-D hormone not only accelerate callus growth but also heighten flavone contents in seccedent culture.(3) Carbon inhibitates callus growth, but enhances flavone accumulation.
Keywords:culture condition  Ginkgo biloba  callus  flavone content
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