植物乳杆菌C88联合人参多糖的免疫调节作用

以环磷酰胺诱导昆明小鼠建立免疫抑制模型后,分别给其灌胃人参多糖、人参多糖联合植物乳杆菌(Lactobacillus plantarum)C88。实验结束后,采用酶联免疫吸附实验检测了小鼠血清中的白细胞介素-2(interleukin-2,IL-2)、白细胞介素-4(interleukin-4,IL-4)、白细胞介素-10(interleukin-10,IL-10)、干扰素-γ(interferon-γ,IFN-γ)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、免疫球蛋白G(immunoglobulin G,Ig G),并利用碳廓清法检测了小鼠吞噬细胞吞噬能力,评价了人参多糖(water-soluble ginseng polysaccharides,WGPA)联合植物乳杆菌C88的体内免疫调节作用。结果表明WGPA联合益生菌C88可明显增强免疫抑制小鼠吞噬细胞的吞噬能力和迟发型超敏反应,显著上调血清中IL-2、IL-4、IFN-γ、TNF-α、Ig G水平,下调IL-10水平,并呈现剂量依赖性,且联合用药组的作用明显强于人参多糖WGPA单独用药组。综上所述,植物乳杆菌C88联合人参多糖可以发挥一定的免疫调节作用。

Immunomodulatory Effects of Ginseng Polysaccharides Combined with Lactobacillus plantarum C88

In this study, a mouse model of cyclophosphamide (CY)-induced immunosuppression was used to evaluate the
immunomodulatory effects of water-soluble ginseng polysaccharides (WGPA) combined with Lactobacillus plantarum
C88. The mice was administered with WGPA alone and combined with L. plantarum C88 by gavage, respectively. The
serum levels of interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-10 (IL-10), interferon-γ (IFN-γ), tumor necrosis
factor-α (TNF-α) and immunoglobulin G (IgG) in mice were measured by enzyme-linked immunosorbent assay. The
phagocytic index of phagocytes was also examined by carbon clearance test. The results showed that WGPA combined with
Lactobacillus plantarum C88 could significantly enhance the phagocytic capacity of phagocytes. IL-2, IL-4, IFN-γ, TNF-α
and IgG levels were significantly increased, whereas IL-10 level was significantly decreased in a dose-dependent manner. In
addition, the immunomodulatory effect of the combined treatment was significantly stronger than that of WGPA alone. In
conclusion, WGPA combined with L. plantarum C88 has certain immunomodulatory effects.