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基于HS-SPME-GC-MS技术对凤凰单丛乌龙茶香气成分比较分析
引用本文:史敬芳,陈 栋,黄文洁,陈 庄,吴华玲,张文虎,贝锦龙,晏石娟.基于HS-SPME-GC-MS技术对凤凰单丛乌龙茶香气成分比较分析[J].食品科学,2016,37(24):111-117.
作者姓名:史敬芳  陈 栋  黄文洁  陈 庄  吴华玲  张文虎  贝锦龙  晏石娟
作者单位:1.广东省农业科学院农业生物基因研究中心,广东 广州 510640; 2.广东省农业科学院茶叶研究所,广东省茶树资源创新利用重点实验室,广东 广州 510640
基金项目:广东省农业领域科技计划项目(2013B020305005);国家现代农业(茶叶)产业技术体系建设专项(CARS-23);广东省农业科学院院长基金项目(201616);广东省农业攻关引导项目(2012B020305011)
摘    要:采用顶空固相微萃取-气相色谱-质谱联用法,对凤凰水仙群体茶树品种和蜜兰香、姜花香、杏仁香、夜来香单丛茶无性品系加工的5 种代表性凤凰单丛乌龙茶成品茶进行香气分析。从凤凰单丛乌龙茶中共鉴定出包括醇类、醛类、酯类、酮类、烯类和烷烃类等55 种香气化合物,其中醇类物质相对含量最高,其次是烯类。研究发现凤凰单丛乌龙茶的共性主导香气成分为脱氢芳樟醇、芳樟醇、芳樟醇氧化物、D-柠檬烯、β-月桂烯、吲哚、茉莉酮、橙花叔醇、苯乙腈、伞花烃。然而,在不同香型的凤凰单丛茶中主导香气化合物的相对含量差别较大。

关 键 词:凤凰单丛乌龙茶  香气化合物  顶空固相微萃取  气相色谱-质谱联用  

Analysis of Flavor Components in Fenghuang Dancong Oolong Teas Using HS-SPME-GC-MS
SHI Jingfang,CHEN Dong,HUANG Wenjie,CHEN Zhuang,WU Hualing,ZHANG Wenhu,BEI Jinlong,YAN Shijuan.Analysis of Flavor Components in Fenghuang Dancong Oolong Teas Using HS-SPME-GC-MS[J].Food Science,2016,37(24):111-117.
Authors:SHI Jingfang  CHEN Dong  HUANG Wenjie  CHEN Zhuang  WU Hualing  ZHANG Wenhu  BEI Jinlong  YAN Shijuan
Affiliation:1. Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 2. Guangdong Key Laboratory of Tea Plant Resources Innovation and Utilization, Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
Abstract:This study aimed to identify and quantify volatile compounds in five different Fenghuang Dancong oolong teas using head space solid-phase micro-extraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). Fiftyfive volatile compounds, including alcohols, aldehydes, esters, ketones, alkenes, and alkanes, were identified. Alcohols were the major components, followed by alkene. The major volatile compounds common to these five types of oolong teas were hotrienol, linalool, linaloloxide, D-limonene, β-myrcene, indole, jasmone, nerolidol, benzyl nitrile and σ-cymene. However, their relative contents were different, which are consistent with their aroma patterns.
Keywords:Fenghuang Dancong oolong tea  volatile compounds  headspace solid-phase micro-extraction (HS-SPME)  gas chromatography-mass spectrometry (GC-MS)  
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