| 莲子红衣多糖的分离纯化及结构表征 |
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| 引用本文： | 高 航,高延芬,徐 虹.莲子红衣多糖的分离纯化及结构表征[J].食品科学,2016,37(15):94-99. |
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| 作者姓名： | 高 航 高延芬 徐 虹 |
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| 作者单位： | 1.北京工商大学 北京市食品添加剂工程技术研究中心，北京食品营养与人类健康高精尖创新中心，北京 100048；2.深圳市农科集团有限公司，广东 深圳 518040 |
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| 摘 要： | 以莲子红衣为实验对象,将其中的多糖进行分离、纯化并表征结构。采用酶提醇析法提取莲子红衣粗多糖,选择DEAE-C阴离子交换树脂和Sephadex G-25凝胶柱层析,从莲子红衣粗多糖中分离纯化得到中性多糖和酸性多糖两种组分。经凝胶渗透色谱测定,中性和酸性多糖的重均分子质量分别为3.78×104 D和4.94×104 D,纯度分别为91.16%和90.24%。中性多糖组分由葡萄糖、木糖、甘露糖、半乳糖4种单糖组成;酸性多糖组分由葡萄糖、木糖、半乳糖、岩藻糖、阿拉伯糖5种单糖组成。红外光谱证明两种多糖中均含有糖类特征吸收峰,且为α构型的吡喃型多糖。核磁共振氢谱检测又进一步证实了两种多糖均为α构型。
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| 关 键 词： | 莲子红衣 多糖 分离纯化 结构表征 |
Separation,Purification and Structural Characteristics of Polysaccharides from Red Skin of Lotus Seeds |
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| GAO Hang,GAO Yanfen,XU Hong.Separation,Purification and Structural Characteristics of Polysaccharides from Red Skin of Lotus Seeds[J].Food Science,2016,37(15):94-99. |
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| Authors: | GAO Hang GAO Yanfen XU Hong |
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| Affiliation: | 1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China; 2. Shenzhen Nongke Group Co. Ltd.,Shenzhen 518040, China |
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| Abstract: | The crude polysaccharides isolated from the red skin of lotus seeds by enzymatic extraction and ethanol
precipitation were separated into neutral and acidic polysaccharides by DEAE-C column chromatography and subsequent
Sephadex G-25 gel column chromatography. Gel permeation chromatography (GPC) analysis showed that the molecular
weights of the neutral and acidic polysaccharides were 3.78 × 104 D and 4.94 × 104 D with a purity of 91.16% and 90.24%,
respectively. The neutral polysaccharide was composed of 4 monosaccharides including glucose and xylose, while the
acidic polysaccharide was mainly composed of 5 monosaccharides including glucose, xylose and galactose. Infrared spectra
preliminarily confirmed that both purified fractions were pyranoses with α configuration. This conclusion was further proved
by nuclear magnetic resonance. |
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| Keywords: | red skin of lotus seeds polysaccharide separation and purification structural characterization |
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