鸡血藤原花青素的纯化及活性评价

采用大孔吸附树脂对鸡血藤原花青素进行纯化，并对原花青素纯度、1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基清除活性及α-葡萄糖苷酶抑制活性进行评价。比较3 种大孔吸附树脂对原花青素静态吸附及解吸附能力，从D101、X-5及AB-8树脂筛选出X-5型树脂用于纯化。对X-5型树脂的动态吸附及解吸附条件进行优化，获得最适条件为：上样质量浓度6.00 mg/mL，上样流速2 BV/h，上样量10 BV，洗脱流速1 BV/h，洗脱剂用量2 BV。利用不同体积分数乙醇洗脱可得到不同纯度的原花青素，其中70%乙醇纯化物原花青素纯度最高，具有最强的DPPH自由基清除活性及α-葡萄糖苷酶抑制活性。相关性分析表明原花青素可能是鸡血藤抗氧化及抑制α-葡萄糖苷酶的主要活性成分。

Purification and Bioactivity Evaluation of Proanthocyanidins from Spatholobi Caulis

Proanthocyanidins from Spatholobi Caulis were purified by macroporous adsorption resin, and then their purity,
1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and α-glucosidase inhibitory activity were evaluated.
In terms of static adsorption and desorption capacity, X-5 resin was chosen as the better sorbent for the purification of
proanthocyanidins compared with D101 and AB-8 resin. Afterwards, the optimal parameters of dynamic adsorption and
desorption for proanthocyanidin purification were obtained as follows: sample concentration, 6.00 mg/mL; loading rate,
2 BV/h; loading amount, 10 BV; eluent flow rate, 1 BV/h; and eluent volume, 2 BV. Proanthocyanidins with different purities were
obtained by elution with different concentrations of ethanol, and the use of 70% ethanol as eluent gave the highest purity and strongest
DPPH radical scavenging activity as well as α-glucosidase inhibitory activity. The correlation analysis indicated that proanthocyanidins
might be the major components responsible for the antioxidant activity and α-glucosidase inhibitory activity of Spatholobi Caulis.