低聚体葡萄籽原花青素对顺铂损伤HEK293细胞及抗癌活性的影响

目的:探讨低聚体葡萄籽原花青素(grape seed proanthocyanidins extract,GSPE)及其结构单元儿茶素(catechin,C)对顺铂(cis-dichlorodiamineplatinum(II),DDP)损伤HEK293人胚肾细胞的保护作用以及对DDP抗A549人肺腺癌细胞活性的影响。方法:体外培养HEK293细胞和A549细胞,以DDP建立损伤模型,分别用不同质量浓度的低聚体GSPE和儿茶素对细胞进行预处理,四甲基偶氮唑蓝(methyl thiazolyl tetrazolium,MTT)法测定细胞抑制率和存活率。结果:当低聚体GSPE质量浓度为16 mg/L时,对DDP诱导的HEK293细胞损伤的保护作用最佳,与DDP损伤组相比有显著差异(P0.05),并且在达到此质量浓度时可显著增强DDP对A549细胞的损伤效果(P0.05)。相同条件下儿茶素对DDP诱导的HEK293细胞损伤无显著影响(P0.05)。结论:体外培养条件下,一定质量浓度的低聚体GSPE对DDP诱导的HEK293细胞损伤具有一定的保护作用,同时能增强DDP对A549细胞的杀伤作用。儿茶素对DDP诱导HEK293细胞损伤的保护作用不明显。

Protective Effect of Grape Seed Oligomeric Proanthocyanidins Extract against Cisplatin-Induced Nephrotoxicity in HEK293 Cell and Effect on Anticancer Activity of Cisplatin in Human Lung Cancer Cells

Objective: To investigate the protective effect of grape seed oligomeric proanthocyanidins extract (GSOPE) and
catechin (C) against cisplatin (DDP)-induced nephrotoxicity in human embryonic kidney (HEK) 293 cells and its effect on
anticancer activity of DDP in human lung cancer A549 cells. Methods: HEK293 cells and A549 cells were cultured in vitro.
The protective effects of GSOPE and catechin at various concentrations on DDP-induced HEK293 cells and their synergistic
interaction with DDP against A549 cells were evaluated by MTT assay. Results: GSOPE at 16 mg/L could significantly
protect DDP-induced HEK239 cells from death (P < 0.05), and the protective effect was better than that of other groups.
GSOPE at 16 mg/L could also significantly enhance DDP-induced A549 cell death (P < 0.05). On the other hand, catechin
had no influence on DDP-induced HEK293 cell death. Conclusion: In vitro, GSOPE, rather than catechin, can protect
DDP-induced nephrotoxicity and can enhance DDP-induced lung cancer cell death.