唾液乳杆菌的分离鉴定及生物特性研究

从健康散养的溜达鸡小肠黏膜中分离出一株乳杆菌，通过菌落形态观察、革兰氏染色、生理生化鉴定以及16S rRNA基因序列测定和同源性分析，确定所分离的菌株为唾液乳杆菌，并命名Lactobacillus salivarius C-1-3。对该菌进行抑菌、纤维素降解、耐酸、耐胆盐、耐高温实验。结果表明：其对常见的致病菌大肠杆菌、沙门氏菌和金黄色葡萄球菌都具有良好的抑制作用，且该菌株能够降解纤维素。经过多次驯化后，该菌株表现出较好的抑菌作用及耐酸、耐高温特性。

Isolation and Identification of Lactobacillus salivarius from Chicken Intestine and Its Biological Characteristics

An isolate of the anaerobic bacterium Lactobacillus salivarius was obtained from the intestine of chicken and
identified by colony morphology, Gram staining, physio-biochemical reactions, and 16S rRNA sequencing analysis. Results
showed that the strain was Lactobacillus salivarius and named as Lactobacillus salivarius C-1-3. Then the strain was
subjected to antibacterial, cellulose degradation, and acid, bile salt and high temperature tolerance tests. It turned out that
the strain C-1-3 had a powerful antibacterial effect against E. coli, Salmonella and Staphylococcus aureus. And it also had
cellulose degradation ability. After several generations of domestication, this strain possessed good antibacterial performance
and significant tolerance to acid and high temperature.