| 南海鸢乌贼墨汁多糖分离纯化及组分分析 |
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| 引用本文： | 黄卉,杨丽芝,杨贤庆,李来好,郝淑贤,魏涯,王锦旭.南海鸢乌贼墨汁多糖分离纯化及组分分析[J].食品科学,2017,38(24):118-123. |
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| 作者姓名： | 黄卉 杨丽芝 杨贤庆 李来好 郝淑贤 魏涯 王锦旭 |
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| 作者单位： | （中国水产科学研究院南海水产研究所，农业部水产品加工重点实验室，国家水产品加工技术研发中心，广东?广州 510300） |
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| 基金项目： | 农业部财政重大专项（NFZX2013）；国家重点基础研究发展计划（973计划）项目（2014CB441500） |
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| 摘 要： | 目的:从南海鸢乌贼墨汁中分离纯化多糖,并分析其多糖组分。方法:利用水提醇沉法得粗多糖,通过固相萃取层析法除色素对多糖进行纯化,纯化多糖依次经DEAE-52离子交换柱、Sephacryl HR-300凝胶柱分级纯化,按峰收集得单一组分,采用1-苯基-3-甲基-5-吡唑啉酮(1-phenyl-3-methyl-5-pyrazolone,PMP)衍生化方法,通过超高效液相色谱分析多糖组分。结果:粗多糖经DEAE-52离子交换柱、Sephacryl HR-300凝胶柱后收集得到3个糖组分,分别为SIP1、SIP2、SIP3;通过PMP衍生化方法分析单糖组成,得:SIP1是由D-甘露糖、D-葡萄糖、D-半乳糖3种单糖缩合而成;SIP2和SIP3分别是由D-甘露糖、鼠李糖、D-葡萄糖、D-半乳糖及L-(-)-岩藻糖5种单糖按照不同比例缩合而成。
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| 关 键 词： | 墨多糖 分离纯化 组分分析 |
Separation,Purification and Monosaccharide Composition of Polysaccharides from the Ink of Sthenoteuthis oualaniensis in South China Sea |
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| HUANG Hui,YANG Lizhi,YANG Xianqing,LI Laihao,HAO Shuxian,WEI Ya,WANG Jinxu.Separation,Purification and Monosaccharide Composition of Polysaccharides from the Ink of Sthenoteuthis oualaniensis in South China Sea[J].Food Science,2017,38(24):118-123. |
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| Authors: | HUANG Hui YANG Lizhi YANG Xianqing LI Laihao HAO Shuxian WEI Ya WANG Jinxu |
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| Affiliation: | (Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, National R&D Center for Aquatic Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China) |
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| Abstract: | Objective: To isolate and purify polysaccharides from the ink of Sthenoteuthis oualaniensis in the South China Sea, and to analyze the monosaccharide composition of the purified polysaccharides. Methods: Crude polysaccharides were isolated through water extraction followed by alcohol precipitation and passed through a solid phase extraction column to remove the pigment. The polysaccharides were fractionated and further purified into homogeneity by sequential chromatographies on DEAE-52 ion exchange and Sephacryl HR-300 columns. The monosaccharide composition was analyzed by ultra performance liquid chromatography (UPLC) after 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization. Results: Three purified polysaccharides designated as SIP1, SIP2, and SIP3 were obtained. SIP1 was composed of D-mannose, D-glucose, and D-galactose while SIP2 and SIP3 were composed of D-mannose, rat sugar, D-glucose, D-galactose and L-(-)-fucose in different ratios. |
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| Keywords: | sepia ink polysaccharides separation and purification monosaccharide analysis |
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