北五味子多糖对RAW264.7细胞的免疫调节作用

北五味子是我国传统中药材,近年来因其具有的护肝、安神等多种功效而得到了广泛地关注和研究。本实验提取北五味子多糖并超滤处理获得不同分子质量的多糖组分Sp1(100 ku)和Sp2(10~100 ku),分别按不同质量浓度对小鼠巨噬细胞系RAW264.7孵育处理,通过检测细胞增殖活性、吞噬能力、NO分泌水平及培养基中细胞因子(细胞白介素(interleukin,IL)-1β、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α))的含量,并与脂多糖实验组进行对比,评价北五味子多糖在免疫调节方面的作用。结果显示,较低分子质量的Sp2组分活性较强,在500.00μg/m L以上时,可以对小鼠巨噬细胞RAW264.7的吞噬能力、NO分泌水平、IL-1β和TNF-α含量有普遍的促进作用,显现出良好的免疫提高潜力,而较高分子质量的Sp1组分活性较弱。

Immunomodulatory Effect of Schisandra chinensis Polysaccharides on RAW264.7 Macrophages

As a traditional Chinese medicinal herb, Schisandra chinensis has been widely concerned among researchers and studied for its functions such as protecting the liver and soothing the nerves in recent years. In this paper, two purified polysaccharides from Schisandra chinensis with different molecular weights, designated Sp1 (>100 ku) and Sp2 (10–100 ku), were obtained by ultrafiltration, and a macrophage cell line RAW264.7 was incubated in the presence of each polysaccharide at different concentrations. The immunomodulatory effect of the two polysaccharides was evaluated based on the proliferation activity of cultured splenocytes as determined by MTT assay, neutral red phagocytosis, NO production and cytokines (interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)) expression levels in comparison with the lipopolysaccharide treatment group. The results showed that the immunomodulatory activity of Sp2 at 500.00 μg/mL was strong, and Sp2 could promote the phagocytosis of macrophages, NO production, and the secretion of IL-1β and TNF-α. But the activity of Sp1 was weak.