高效液相色谱法同时测定工夫红茶中10种内含物成分

建立高效液相色谱法同时测定工夫红茶中咖啡碱、儿茶素、表儿茶素、表没食子儿茶素、表儿茶素没食子酸酯、表没食子儿茶素没食子酸酯、茶黄素、茶黄素-3-没食子酸酯、茶黄素-3’-没食子酸酯、茶黄素双没食子酸酯10种内含物成分含量的方法。通过色谱条件优化,确定出工夫红茶中10种内含物成分含量测定的最佳色谱条件为:色谱柱为Whatman Partisphere C_(18)液相色谱柱(4.6 mm×125 mm,5μm)、柱温32℃、流动相为0.035%三氟乙酸-5%乙腈溶液和0.025%三氟乙酸-50%乙腈溶液、流速1 m L/min、进样量10μL、检测波长205 nm。在此条件下,上述10种内含物成分得到很好的分离和测定。各种内含物的线性范围为0.05~500μg/m L,标准曲线相关系数均在0.999 8以上,精密度检测相对标准偏差为0.72%~3.32%(n=5),重复性检测相对标准偏差为1.87%~4.43%(n=5),回收率在94.73%~102.38%之间。该方法简单、快捷、准确、重复性好,可应用于工夫红茶中10种内含物质的同时快速测定。

Determination of Ten Components in Congou Black Tea by HPLC

A high performance liquid chromatographic (HPLC) method was established for the simultaneous determination
of ten components including caffeine, catechin (C), epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG),
epigallocatechin gallate (EGCG), theaflavin (TF1), theaflavin-3-gallate (TF2A), theaflavin-3’-gallate (TF2B), theaflavin-
3,3’-digallate (TF3), in Congou black tea. The chromatographic conditions were optimized. A Whatman Partisphere C18
column (4.6 mm × 125 mm, 5 μm) was used to separate the ten compounds. The mobile phase was a mixture of 0.035%
trifluoroacetic acid containing 5% acetonitrile (A) and 50% acetonitrile solution containing 0.025% trifluoroacetic acid and
methanol solution (B) at a flow rate of 1 mL/min. The column temperature and UV detection wavelength were set as 32 ℃
and 205 nm, respectively. The ten components were successfully separated and detected under the chosen experimental
conditions. The calibration curves for all these components displayed a good linear relationship with correlation coefficients
above 0.999 8 and precision relative standard deviations (RSDs) of 0.72%–3.32% (n = 5) and repeatability RSD of 1.87%–
4.43% (n = 5). The recovery rates of the ten compounds were in the range of 94.73%–102.38%. The method is convenient,
rapid, accurate, reproducible and applicable to simultaneously determine these ten components in Congou black tea.