| 响应面法优化Bacillus thuringiensis ZJOU-010产壳聚糖酶 |
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| 引用本文： | 陈静,陈小娥,方旭波,余辉.响应面法优化Bacillus thuringiensis ZJOU-010产壳聚糖酶[J].食品科学,2011(5). |
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| 作者姓名： | 陈静 陈小娥 方旭波 余辉 |
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| 作者单位： | 浙江海洋学院食品与药学学院医学院; |
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| 基金项目： | 浙江省自然科学基金项目(Y305119); 浙江省优先主题农业项目(2008C12029); 浙江海洋学院科研启动基金项目 |
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| 摘 要： | 目前,利用壳聚糖酶降解壳聚糖已成为生产功能性壳寡糖的首选途径。本研究在单因素试验的基础上,通过响应面法结合中心组合设计优化了Bacillus thuringiensis ZJOU-010产壳聚糖酶的培养条件。结果表明:当虾壳粉质量浓度为44.96g/L、(NH4)2SO4质量浓度为1.48g/L、pH值为6.78时,B.thuringiensis ZJOU-010的壳聚糖酶活力达到4.25U/mL。优化后获得的实验值与模型的预测值(4.16U/mL)基本相符,且较优化前的酶活力(3.59U/mL)提高了18.47%。研究表明,利用生物催化技术以虾加工企业的副产物——虾壳粉为唯一碳源和氮源生产壳寡糖是可行的。
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| 关 键 词： | 壳寡糖 虾壳粉 壳聚糖酶 优化 响应面 |
Optimization of Chitosanase Production from Bacillus thuringiensis ZJOU-010 by Response Surface Methodology |
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| CHEN Jing,CHEN Xiao-e,FANG Xu-bo,YU Hui.Optimization of Chitosanase Production from Bacillus thuringiensis ZJOU-010 by Response Surface Methodology[J].Food Science,2011(5). |
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| Authors: | CHEN Jing CHEN Xiao-e FANG Xu-bo YU Hui |
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| Affiliation: | CHEN Jing,CHEN Xiao-e*,FANG Xu-bo,YU Hui(College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316000,China) |
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| Abstract: | Chitooligosaccharides exhibit a broad range of potential applications in the fields of biomedicine,pharmaceuticals,food,cosmetics and agriculture.In the present study,based on the results from single factor tests,response surface methodology(RSM) combined with central composite design was used to optimize cultivation conditions of Bacillus thuringiensis ZJOU-010 for producing chitosanase.The optimal conditions were achieved to be shrimp shell powder(SSP) concentration of 44.96 g/L,(NH4)2SO4 concentration of... |
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| Keywords: | chitooligosaccharides shrimp shell powder chitosanase optimization response surface methodology |
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