响应面法优化金针菇抗氧化物质的超声波辅助提取工艺

目的:采用超声波辅助提取法从金针菇中提取抗氧化物质,利用响应曲面法对提取工艺参数进行优化,得出最优工艺参数。方法:采用DPPH自由基法检测提取物的清除自由基能力,选择对清除率有显著影响的3个因素——乙醇体积分数、超声功率、提取时间,做三因素三水平响应面分析试验。结果:通过典型性分析得出最优工艺为乙醇体积分数95%、超声频率40kHz、超声功率300W、提取时间20.24min。在此条件下所得提取物DPPH自由基清除率达到了85.32%。结论:超声波辅助提取的金针菇抗氧化物质具有较高清除率,响应曲面法优化提取金针菇抗氧化物质条件参数可行。

Optimization of Ultrasonic-assisted Extraction of Antioxidants from the Fruiting Body of Flammulina velutipes by Response Surface Analysis

Objective:Ultrasonic treatment was introduced to the ethanolic extraction of antioxidants from the fruiting body of Flammulina velutipes with the aim of enhancing cell disruption,and the extraction process was optimized by response surface methodology. Methods:For the evaluation of the ability of extract to scavenge free radicals,a DPPH free radical system was constructed. Ethanol concentration,ultrasonic power and extraction time were selected as major factors that influence DPPH radical scavenging ratio,a...