| 响应面试验优化莲蓬壳总黄酮超声提取条件及其抗氧化活性 |
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| 引用本文： | 胡卫成,王新风,沈 婷,王毓宁,陈 铭,尤 龙,纪丽莲,李鹏霞.响应面试验优化莲蓬壳总黄酮超声提取条件及其抗氧化活性[J].食品科学,2015,36(24):51-56. |
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| 作者姓名： | 胡卫成 王新风 沈 婷 王毓宁 陈 铭 尤 龙 纪丽莲 李鹏霞 |
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| 作者单位： | 1.淮阴师范学院 江苏省高校区域现代农业与环境保护协同创新中心，淮阴师范学院生命科学学院，江苏省环洪泽湖生态农;
业生物技术重点实验室，江苏 淮安 223300；2.江苏省农业科学院农产品加工研究所，江苏 南京 210014 |
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| 基金项目： | 江苏省农业科技自主创新资金项目（CX（13）3079） |
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| 摘 要： | 对莲蓬壳总黄酮的超声提取工艺和抗氧化活性进行研究。在单因素试验基础上,采用响应面分析法对超声辅助提取的工艺参数进行优化,得到最优工艺条件为液料比40∶1(m L/g)、乙醇体积分数48%、提取温度60℃、提取时间10 min。在此条件下测得莲蓬壳总黄酮提取率为8.32%。抗氧化实验结果表明:莲蓬壳总黄酮具有较强清除1,1-二苯基-2-三硝基苯肼自由基能力和还原力,同时可显著性抑制H2O2诱导的人皮肤纤维细胞氧化应激损伤,是一种极具开发潜力的天然抗氧化剂。
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| 关 键 词： | 响应面分析法 莲蓬壳 总黄酮 超声提取 自由基 |
Optimization of the Extraction Process for Flavonoids from Lotus (Nelumbo nucifera Gaertn.) Receptacle and Their Antioxidant Activities |
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| HU Weicheng,WANG Xinfeng,SHEN Ting,WANG Yuning,CHEN Ming,YOU Long,JI Lilian,LI Pengxia.Optimization of the Extraction Process for Flavonoids from Lotus (Nelumbo nucifera Gaertn.) Receptacle and Their Antioxidant Activities[J].Food Science,2015,36(24):51-56. |
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| Authors: | HU Weicheng WANG Xinfeng SHEN Ting WANG Yuning CHEN Ming YOU Long JI Lilian LI Pengxia |
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| Affiliation: | 1. Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Jiangsu Collaborative Innovation Center of Regional;
Modern Agriculture & Environmental Protection, Huaiyin Normal University, School of Life Science, Huaiyin Normal University,;
Huai’an 223300, China; 2. Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China |
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| Abstract: | Using Box-Behnken design and response surface methodology, the optimized extraction conditions for maximum
yield (83.2 mg rutin equivalents/g) of total flavonoids from lotus (Nelumbo nucifera Gaertn.) receptacle were determined
as follows: temperature, 60 ℃; ethanol concentration, 48%; ratio of liquid to solid, 40:1 (mL/g); and extraction duration,
10 min. The flavonoids extracted from lotus receptacle (FLR) exerted remarkable scavenging effects on 1,1-diphenyl-2-
picrylhydrazyl (DPPH) free radical and exhibited high reducing power. Moreover, FLR pretreatment effectively elevated cell
viability in H2O2-treated human skin fibroblast cells. The results obtained from in vitro models clearly suggest that FLR, as a
byproduct of the agricultural and food industries, is a potential source of natural antioxidant. |
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| Keywords: | response surface methodology lotus receptacle total flavnoids ultrasound-assisted extraction free radicals |
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